Protein repellent anti-coagulative mixed-charged cellulose derivative coatings

• Published in: Carbohydrate polymers Vol. 254; p. 117437
• Main Authors: Bračič, Matej, Mohan, Tamilselvan, Kargl, Rupert, Grießer, Thomas, Heinze, Thomas, Stana Kleinschek, Karin
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.02.2021
• Subjects: Amination; Amino cellulose; Anticoagulants - chemistry; Anticoagulants - pharmacology; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Blood Coagulation - drug effects; Cellulose - analogs & derivatives; Cellulose - pharmacology; Cellulose sulphate; Coagulation; Fibrinogen; Humans; Hydrogen-Ion Concentration; Kinetics; Membranes, Artificial; Multilayer; Plasma; Polyelectrolyte; Polyelectrolytes - chemistry; Polyesters - chemistry; Protein Binding - drug effects; Protons; QCM-D; Serum albumin; Serum Albumin, Bovine - chemistry; Static Electricity; Sulfuric Acid Esters - chemistry; Thrombin - antagonists & inhibitors; Thrombin - metabolism; Water - chemistry; XPS; Polyelectrolyte; Plasma; Multilayer; Fibrinogen; Coagulation; QCM-D; Amino cellulose; Cellulose sulphate; Serum albumin; XPS
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2020.117437

•Sulphated and aminated celluloses form stable mixed-charge complexes on polycaprolactone.•Dry and wet masses of coatings can be measured by QCM-D.•Charge complexes strongly reduce albumin binding.•Amino cellulose coatings and charge complexes retard fibrin clot formation. This study describes the formation of cellulose based polyelectrolyte charge complexes on the surface of biodegradable polycaprolactone (PCL) thin films. Anionic sulphated cellulose (CS) and protonated cationic amino cellulose (AC) were used to form these complexes with a layer-by-layer coating technique. Both polyelectrolytes were analyzed by charge titration methods to elucidate their pH-value dependent protonation behavior. A quartz crystal microbalance with dissipation (QCM-D) in combination with X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) were used to follow the growth, stability and water content of up to three AC/CS bi-layers in aqueous environment. This was combined with coagulation studies on one, two and three bilayers of AC/CS, measuring the thrombin formation rate and the total coagulation time of citrated blood plasma with QCM-D. Stable mixed charged bilayers could be prepared on PCL and significantly higher masses of AC than of CS were present in these complexes. Strong hydration due to the presence of ammonium and sulphate substituents on the backbone of cellulose led to a significant BSA repellent character of three bilayers of AC/CS coatings. The total plasma coagulation time was increased in comparison to neat PCL, indicating an anticoagulative nature of the coatings. Surprisingly, a coating solely composed of an AC layer significantly prolonged the total coagulation time on the surfaces although it did not prevent fibrinogen deposition. It is suggested that these cellulose derivative-based coatings can therefore be used to prevent unwanted BSA deposition and fibrin clot formation on PCL to foster its biomedical application.