A GC–MS method for the detection and quantitation of ten major drugs of abuse in human hair samples

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1047; pp. 141 - 150
• Main Authors: Orfanidis, A., Mastrogianni, O., Koukou, A., Psarros, G., Gika, H., Theodoridis, G., Raikos, N.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.03.2017
• Subjects: Derivatization; Drugs of abuse; Gas Chromatography-Mass Spectrometry - methods; GC–MS; Hair - chemistry; Hair analysis; Humans; Limit of Detection; Street Drugs - analysis; Substance Abuse Detection - methods; GC–MS; Hair analysis; Drugs of abuse; Derivatization
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2016.11.011

•Detection and determination of 10 major drugs of abuse in hair sample for forensic applications.•Optimization of derivatization procedure for GC–MS analysis.•Application in hair samples from human subjects drug addicts. A sensitive analytical method has been developed in order to identify and quantify major drugs of abuse (DOA), namely morphine, codeine, 6-monoacetylmorphine, cocaine, ecgonine methyl ester, benzoylecgonine, amphetamine, methamphetamine, methylenedioxymethamphetamine and methylenedioxyamphetamine in human hair. Samples of hair were extracted with methanol under ultrasonication at 50°C after a three step rinsing process to remove external contamination and dirt hair. Derivatization with BSTFA was selected in order to increase detection sensitivity of GC/MS analysis. Optimization of derivatization parameters was based on experiments for the selection of derivatization time, temperature and volume of derivatising agent. Validation of the method included evaluation of linearity which ranged from 2 to 350ng/mg of hair mean concentration for all DOA, evaluation of sensitivity, accuracy, precision and repeatability. Limits of detection ranged from 0.05 to 0.46ng/mg of hair. The developed method was applied for the analysis of hair samples obtained from three human subjects and were found positive in cocaine, and opiates.