Electrochemical nano-genosensor for highly sensitive detection of miR-21 biomarker based on SWCNT-grafted dendritic Au nanostructure for early detection of prostate cancer

• Published in: Talanta (Oxford) Vol. 209; p. 120595
• Main Authors: Sabahi, Abbas, Salahandish, Razieh, Ghaffarinejad, Ali, Omidinia, Eskandar
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2020
• Subjects: Dendritic gold nanostructures; Electrochemical nano-genosensor; miRNA-21; Oligonucleotide; Prostate cancer; Single wall carbon nanotubes; Oligonucleotide; Dendritic gold nanostructures; Electrochemical nano-genosensor; Prostate cancer; miRNA-21; Single wall carbon nanotubes
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2019.120595

MicroRNAs (miRNAs) appear as a novel reliable candidate in biomarkers for early diagnosis of cancer. Due to their roles in various types of cancer, their potential as a diagnostic biomarker is getting more attention. Here, a novel electrochemical biosensor for detection of miR-21 was demonstrated, through combining the advantages of electrochemical methods and nanomaterials with the selectivity of oligonucleotides, based on thiolated receptor probe-functionalized dendritic gold nanostructures (den-Au) via the self-assembly monolayer (SAM) process which grafted on the single-wall carbon nanotubes (SWCNTs) platform on the surface of the fluorine-doped tin oxide (FTO) electrode. Cadmium ions (Cd2+) were used as signal units and also signal amplification substance which labeled before on miR-21 target. The oxidation signal of Cd2+ as a signal unit was measured by differential pulse voltammetry (DPV) technique that had a very wide linear relationship with the concentration of miR-21 target (0.01 fmol L−1 to 1 μmol L−1) and low experimental detection limit of 0.01 fmol L−1. Furthermore, fabricated biosensor showed acceptable performance in human serum samples and also good selectivity indiscriminate between the complementary target and non-complementary one, so this nano-genosensor can clinically be used for prostate cancer diagnosis through the detection of miR-21 in human serum samples. [Display omitted] •A new nano-genesensor was introduced for early diagnosis of prostate cancer.•miR-21 as a biomarker of prostate cancer was measured.•The nano-genosensor shows high recovery and very wide linear range.•The biosensor was successfully applied in human serum sample.