Inter-laboratory skin distribution study of 4-n-butyl resorcinol: The importance of liquid chromatography/mass spectrometry (HPLC–MS/MS) bioanalytical validation

In the present study, three laboratories independently compared percutaneous absorption and distribution of 4-n-butylresorcinol, using human skin from five donors. Each laboratory used the same protocol for percutaneous absorption studies but different LC–MS/MS analytical methods to quantify the tes...

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Bibliographic Details
Published inJournal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1060; pp. 416 - 423
Main Authors Wargniez, William, Jungman, Elsa, Wilkinson, Simon, Seyler, Nathalie, Grégoire, Sébastien
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 15.08.2017
Subjects
Chromatography, High Pressure Liquid - methods
Chromatography, High Pressure Liquid - standards
Humans
Limit of Detection
Linear Models
Mass spectrometry
Reproducibility of Results
Research Design - standards
Resorcinols - analysis
Resorcinols - metabolism
Resorcinols - pharmacokinetics
robustness
Skin absorption
Skin Absorption - physiology
Solubility
Stratum corneum tape stripping
Tandem Mass Spectrometry - methods
Tandem Mass Spectrometry - standards
Validation
LLOQ
APCI
Ep
SC
SD
QC
W
ME
Skin absorption
ESI
ULOQ
LOD
Validation
De
TEWL
AD
MS
robustness
IS
ACN
ER
CV
RF
MeOH
Stratum corneum tape stripping
LC
FA
Mass spectrometry
TS
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Summary:In the present study, three laboratories independently compared percutaneous absorption and distribution of 4-n-butylresorcinol, using human skin from five donors. Each laboratory used the same protocol for percutaneous absorption studies but different LC–MS/MS analytical methods to quantify the test compound. All laboratories respected the mass balance criteria (i.e. 100±15%; average 96.5–102% of applied dose). Regarding usual inter-lab variability, good agreement was observed for all compartments with the greatest difference in the epidermis: 3.3 fold increase. The data obtained demonstrate that robustness of skin absorption data rely on properly validated analytical methods including sample extraction and LC–MS/MS method. It also includes clearly defined cutaneous absorption protocol for dose skin preparation, application, washing and tape stripping.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2017.05.026