Routine micromethod for the determination of vitamin K1 in human plasma

• Published in: Therapeutic drug monitoring Vol. 35; no. 6; p. 859
• Main Authors: Meineke, Ingolf, Willnow, Cornelia, Stingl, Julia, Brockmöller, Jürgen
• Format: Journal Article
• Language: English
• Published: United States 01.12.2013
• Subjects: Animals; Antifibrinolytic Agents - blood; Calibration; Cattle; Chromatography, High Pressure Liquid - methods; Humans; Quality Control; Sensitivity and Specificity; Spectrometry, Fluorescence - methods; Vitamin K 1 - blood
• ISSN: 1536-3694
• DOI: 10.1097/FTD.0b013e31828eff38

A selective and sensitive high-performance liquid chromatographic method with fluorescence detection after postcolumn reduction is described for the routine measurement of vitamin K1 in plasma samples of 100 µL of volume. Liquid-liquid extraction was used for the sample preparation with vitamin K2 as an internal standard. For the chromatographic separation, a standard C18 column was applied. The calibration range used was from 2 to 500 ng/mL. At the low level (5 ng/mL), a coefficient of variation of 14.9% was observed interday in the quality control samples, whereas at the intermediate (50 ng/mL) and high (200 ng/mL) levels such of 6.8% and 8.7% were found. The method has proven robust and convenient for the analysis of samples from clinical pharmacological studies.