Effects of Pu-erh ripened tea on hyperuricemic mice studied by serum metabolomics

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1068-1069; pp. 149 - 156
• Main Authors: Zhao, Ran, Chen, Dong, Wu, Hualing
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.11.2017
• Subjects: Allopurinol - pharmacology; Animals; Biomarkers - blood; Biomarkers - metabolism; Drugs, Chinese Herbal - pharmacology; Fermentation; GC–MS; Hyperuricemia; Hyperuricemia - chemically induced; Hyperuricemia - metabolism; Male; Metabolome - drug effects; Metabolomics; Mice; Pu-erh ripened tea; Tea; Uric Acid - blood; Uric Acid - metabolism; Weight Gain - drug effects; Metabolomics; GC–MS; Hyperuricemia; Pu-erh ripened tea
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2017.10.002

•Serum metabolomics based on GC–MS was employed in this study.•Twelve potential biomarkers associated with hyperuricemia were identified.•Pu-erh ripened tea caused anti-hyperuricemia in mice.•A mechanism of Pu-erh ripened tea effects may be modifying amino acid metabolism. To evaluate effects of Pu-erh ripened tea in hyperuricemic mice, a mouse hyperuricemia model was developed by oral administration of potassium oxonate for 7 d. Serum metabolomics, based on gas chromatography–mass spectrometry, was used to generate metabolic profiles from normal control, hyperuricemic and allopurinol-treated hyperuricemic mice, as well as hyperuricemic mice given Pu-erh ripened tea at three doses. Pu-erh ripened tea significantly lowered serum uric acid levels. Twelve potential biomarkers associated with hyperuricemia were identified. Pu-erh ripened tea and allopurinol differed in their metabolic effects in the hyperuricemic mice. Levels of glutamic acid, indolelactate, L-allothreonine, nicotinoylglycine, isoleucine, l-cysteine and glycocyamine, all involved in amino acid metabolism, were significantly changed in hyperuricemic mice treated Pu-erh ripened tea. Thus, modulating amino acid metabolism might be the primary mechanism of anti-hyperuricemia by Pu-erh ripened tea.