Effects of Pu-erh ripened tea on hyperuricemic mice studied by serum metabolomics
•Serum metabolomics based on GC–MS was employed in this study.•Twelve potential biomarkers associated with hyperuricemia were identified.•Pu-erh ripened tea caused anti-hyperuricemia in mice.•A mechanism of Pu-erh ripened tea effects may be modifying amino acid metabolism. To evaluate effects of Pu-...
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Published in | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1068-1069; pp. 149 - 156 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
15.11.2017
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Subjects | |
Online Access | Get full text |
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Summary: | •Serum metabolomics based on GC–MS was employed in this study.•Twelve potential biomarkers associated with hyperuricemia were identified.•Pu-erh ripened tea caused anti-hyperuricemia in mice.•A mechanism of Pu-erh ripened tea effects may be modifying amino acid metabolism.
To evaluate effects of Pu-erh ripened tea in hyperuricemic mice, a mouse hyperuricemia model was developed by oral administration of potassium oxonate for 7 d. Serum metabolomics, based on gas chromatography–mass spectrometry, was used to generate metabolic profiles from normal control, hyperuricemic and allopurinol-treated hyperuricemic mice, as well as hyperuricemic mice given Pu-erh ripened tea at three doses. Pu-erh ripened tea significantly lowered serum uric acid levels. Twelve potential biomarkers associated with hyperuricemia were identified. Pu-erh ripened tea and allopurinol differed in their metabolic effects in the hyperuricemic mice. Levels of glutamic acid, indolelactate, L-allothreonine, nicotinoylglycine, isoleucine, l-cysteine and glycocyamine, all involved in amino acid metabolism, were significantly changed in hyperuricemic mice treated Pu-erh ripened tea. Thus, modulating amino acid metabolism might be the primary mechanism of anti-hyperuricemia by Pu-erh ripened tea. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1570-0232 1873-376X |
DOI: | 10.1016/j.jchromb.2017.10.002 |