Endothelin Receptor-mediated Responses in Trigeminal Ganglion Neurons

• Published in: Journal of dental research Vol. 92; no. 4; pp. 335 - 339
• Main Authors: Yamamoto, T., Ono, K., Hitomi, S., Harano, N., Sago, T., Yoshida, M., Nunomaki, M., Shiiba, S., Watanabe, S., Nakanishi, O., Inenaga, K.
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.04.2013; SAGE PUBLICATIONS, INC
• Subjects: Animals; Calcium (intracellular); Calcium imaging; Calcium mobilization; Cancer; Capsaicin; Capsaicin receptors; Chelerythrine; Dentistry; Dorsal root ganglia; Endothelin 1; Endothelin receptors; Endothelins; Evoked Potentials - physiology; Experiments; Fluorescent Antibody Technique; Immunofluorescence; Male; Membrane Potentials - physiology; Neurons; Neurons - cytology; Neurons - metabolism; Pain; Pain perception; Patch-Clamp Techniques; Pharmaceutical industry; Protein kinase C; Rats; Rats, Wistar; Receptor, Endothelin A - metabolism; Receptor, Endothelin B - metabolism; Studies; Trigeminal ganglion; Trigeminal Ganglion - cytology; Trigeminal Ganglion - physiology; Trigeminal nerve; United States > US; Japan; PKC; BQ-788; TRPV1; endothelin-1; endothelin-3; BQ-123
• ISSN: 0022-0345; 1544-0591
• DOI: 10.1177/0022034513478428

Recent evidence implicates endothelin in nociception, but it is unclear how endothelin activates trigeminal ganglion (TRG) neurons. In the present study, we investigated the expression of the endothelin receptors ETA and ETB and endothelin-induced responses in rat TRG neurons. Double-immunofluorescence studies demonstrated that ETA and ETB were expressed in TRG neurons and that 26% of ETA- or ETB-expressing neurons expressed both receptors. During whole-cell patch-clamp recording, endothelin-1 enhanced an induced current in response to capsaicin, a TRPV1 agonist, in approximately 20% of dissociated neurons. The enhancement was blocked by the PKC inhibitor chelerythrine and by the ETA antagonist BQ-123, but not by the ETB antagonist BQ-788. Ca2+-imaging showed that endothelin-1 increased the intracellular Ca2+ concentration in more than 20% of the dissociated neurons. Importantly, unlike the effect of endothelin-1 on capsaicin-induced current, the Ca2+ response was largely suppressed by BQ-788 but not by BQ-123. These results suggest that ETA-mediated TRPV1 hyperactivation via PKC activation and ETB-mediated Ca2+ mobilization occurs in different subsets of TRG neurons. These endothelin-induced responses may contribute to the induction of orofacial pain. The ETB-mediated function in TRG neurons is a special feature in the trigeminal system because of no ETB expression in dorsal root ganglion neurons.