Metabolomic fingerprinting of Brazilian marine sponges: a case study of Plakinidae species from Fernando de Noronha Archipelago
Marine sponges from the Plakinidae family are well known for hosting cytotoxic secondary metabolites and the Brazilian Atlantic coast and its oceanic islands have been considered as a hotspot for the discovery of new Plakinidae species. Herein, we report the chemical profile among cytotoxic extracts...
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Published in | Analytical and bioanalytical chemistry Vol. 413; no. 16; pp. 4301 - 4310 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.07.2021
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Marine sponges from the Plakinidae family are well known for hosting cytotoxic secondary metabolites and the Brazilian Atlantic coast and its oceanic islands have been considered as a hotspot for the discovery of new Plakinidae species. Herein, we report the chemical profile among cytotoxic extracts obtained from four species of Plakinidae, collected in Fernando de Noronha Archipelago (PE, Northeastern Brazil). Crude organic extracts of
Plakinastrella microspiculifera
,
Plakortis angulospiculatus
,
Plakortis insularis
, and
Plakortis petrupaulensis
showed strong antiproliferative effects against two different cancer cell lines (HCT-116: 86.7–100%; MCF-7: 74.9–89.5%) at 50 μg/mL, by the MTT assay. However, at a lower concentration (5 μg/mL), high variability in inhibition of cell growth was observed (HCT-116: 17.3–68.7%; MCF-7: 0.00–55.5%), even within two samples of
Plakortis insularis
which were collected in the west and east sides of the Archipelago. To discriminate the chemical profile, the samples were investigated by UHPLC-HRMS under positive ionization mode. The produced data was uploaded to the Global Natural Products Social Molecular Networking and organized based on spectral similarities for purposes of comparison and annotation. Compounds such as dipeptides, nucleosides and derivatives, polyketides, and thiazine alkaloids were annotated and metabolomic differences were perceived among the species. To the best of our knowledge, this is the first assessment for cytotoxic activity and chemical profiling for
Plakinastrella microspiculifera
,
Plakortis insularis
and
Plakortis petrupaulensis
, revealing other biotechnologically relevant members of the Plakinidae family.
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1618-2642 1618-2650 |
DOI: | 10.1007/s00216-021-03385-6 |