Highly selective detection of l-Phenylalanine by molecularly imprinted polymers coated Au nanoparticles via surface-enhanced Raman scattering

• Published in: Talanta (Oxford) Vol. 211; p. 120745
• Main Authors: Zhou, Jiayuan, Sheth, Sujitraj, Zhou, Haifeng, Song, Qijun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2020
• Subjects: AuNPs; l-phenylalanine; MIP-AuNPs sensor; Molecularly imprinted polymers; Surface-enhanced Raman scattering (SERS); Molecularly imprinted polymers; Surface-enhanced Raman scattering (SERS); MIP-AuNPs sensor; l-phenylalanine; AuNPs
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2020.120745

Molecularly imprinted film coated gold nanoprticles (MIP-AuNPs) were employed as surface-enhanced Raman scattering (SERS) substrate for sensitive and selective recognition and quantification of l-Phenylalanine (L-Phe). The MIP was in-situ formed on the AuNPs by sol-gel technique using L-Phe as the template molecule, tetraethyl orthosilicate as the crosslinker and phenyltrimethoxysilane as the functional monomer. The efficient removal of template was achieved by ultrasonic treatment. The as-prepared MIP-AuNPs sensor showed a good linear relationship with the concentration of L-Phe in the range of 1.0 × 10−8–1.0 × 10−4 mol L−1 with a limit of detection as low as 1.0 nmol L−1. The sensor also showed an excellent selectivity as L-Phe can be determined in the presence of other amino acid analogues, D-Phe and bovine serum. The stability of the MIP-AuNPs was manifested by the low deviation (RSD = 3.7%) for 40 days subsequent measurements. [Display omitted] •The MIPs functionalized AuNPs were used as a SERS substrate for sensitive and selective detection of l-Phenylalanine.•The efficient removal of template was achieved by ultrasonic treatment.•The thickness of MIPs on AuNPs was tuned in-situ by controlling the amount of precursors to achieve best SERS performance.•l-Phenylalanine was successfully detected with the probe in the coexistence of protein.