Molecular mechanisms of selenite reduction by Lactiplantibacillus plantarum BSe: An integrated genomic and transcriptomic analysis

• Published in: Journal of hazardous materials Vol. 468; p. 133850
• Main Authors: Zhong, Bin, Xu, Weijun, Gong, Ming, Xian, Wei, Xie, Hanyi, Wu, Zhenqiang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.04.2024
• Subjects: Azoreductase; Electron Transport; Genomics; Lactiplantibacillus plantarum; Multiomics; Oxidation-Reduction; Selenious Acid - metabolism; Selenite reduction; Selenium - metabolism; Selenium nanoparticles; SDR; QS; Selenite reduction; EPS; GST; Multiomics; L. plantarum BSe; GR; Se; Se10; Azoreductase; ETSA; Se1; Selenium nanoparticles; Se0; SeNPs; TrxR; Lactiplantibacillus plantarum
• ISSN: 0304-3894; 1873-3336; 1873-3336
• DOI: 10.1016/j.jhazmat.2024.133850

The reduction of selenite [Se(Ⅳ)] by microorganisms is a green and efficient detoxification strategy. We found that Se(Ⅳ) inhibited exopolysaccharide and protein secretion by Lactiplantibacillus plantarum BSe and compromised cell integrity. In this study, L. plantarum BSe reduced Se(Ⅳ) by increasing related enzyme activity and electron transfer. Genomic analysis demonstrated that L. plantarum BSe should be able to reduce Se(Ⅳ). Further transcriptome analysis showed that L. plantarum BSe enhanced its tolerance to Se(Ⅳ) by upregulating the expression of surface proteins and transporters, thus reducing the extracellular Se(Ⅳ) concentration through related enzymatic reactions and siderophore-mediated pathways. Lactiplantibacillus plantarum BSe was able to regulate the expression of related genes involved in quorum sensing and a two-component system and then select appropriate strategies for Se(Ⅳ) transformation in response to varying environmental Se(Ⅳ) concentrations. In addition, azo reductase was linked to the reduction of Se(Ⅳ) for the first time. The present study established a multipath model for the reduction of Se(Ⅳ) by L. plantarum, providing new insights into the biological reduction of Se(Ⅳ) and the biogeochemical cycle of selenium. [Display omitted] •Se(Ⅳ) compromised cell integrity of L. plantarum BSe and inhibited EPS secretion.•BSe reduces Se(Ⅳ) by improving enzyme activity and electron transfer.•BSe upregulated surface proteins and transporters to resist the Se(Ⅳ) stress.•Azo reductase was found to be related to the reduction of Se(Ⅳ) for the first time.