role of carbohydrate in the glycoenzyme invertase of Neurospora crassa

Data obtained concerning the carbohydrate moieties of the glycoenzyme invertase (EC 3.2.1.26, beta-D-fructofuranoside fructohydrolase) from Neurospora crassa were consistent with a linkage of some carbohydrate chains by O-glycosidic bonds to serine and threonine residues; the possibility of N-glycos...

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Bibliographic Details
Published inCanadian journal of biochemistry Vol. 55; no. 3; pp. 249 - 256
Main Authors Tashiro, Y, Trevithick, J.R
Format Journal Article
LanguageEnglish
Published Canada 01.03.1977
Subjects
Amino Acids - analysis
Carbohydrates - analysis
fungi
Glycosides
Neurospora - enzymology
Neurospora crassa - enzymology
Periodic Acid
Pronase
Protein Conformation
Serine
Sucrase - analysis
Threonine
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Summary:Data obtained concerning the carbohydrate moieties of the glycoenzyme invertase (EC 3.2.1.26, beta-D-fructofuranoside fructohydrolase) from Neurospora crassa were consistent with a linkage of some carbohydrate chains by O-glycosidic bonds to serine and threonine residues; the possibility of N-glycosylamine linkage of some of the carbohydrate to the amide group of asparagine is also indicated. The invertase was remarkably stable on storage at low temperatures. Oxidation of the carbohydrate residues in the enzyme by sodium periodate markedly affected the heat-stability of the enzyme. It is suggested that the carbohydrate moieties function as stabilizers of the tertiary structure of the glycoenzyme.
ISSN:0008-4018
DOI:10.1139/o77-035