Selective nano-sensing approach for the determination of inorganic phosphate in human urine samples
A novel sensing approach is presented for the analysis of phosphate ions in human urine samples. The new sensor is based on the fluorescence energy transfer between a luminescent probe ([Tb-EDTA]−1) and gold nanoparticles capped with a cetyltrimethylammonium bromide (Au NPs-CTAB). The strong affinit...
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Published in | Talanta (Oxford) Vol. 164; pp. 209 - 215 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.03.2017
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Subjects | |
Online Access | Get full text |
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Summary: | A novel sensing approach is presented for the analysis of phosphate ions in human urine samples. The new sensor is based on the fluorescence energy transfer between a luminescent probe ([Tb-EDTA]−1) and gold nanoparticles capped with a cetyltrimethylammonium bromide (Au NPs-CTAB). The strong affinity between CTAB receptors and phosphate ions results in a highly selective assay with minimum sample preparation steps. Possible chemical interference is monitored on real-time basis via luminescence lifetime analysis. The simplicity of analysis and the competitive limit of detection in the micro-molar concentration range provide a well-suited approach for routine monitoring of phosphate ions in numerous urine samples.
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•Fluorescence energy transfer for direct sensing of phosphate ions in urine samples.•Micro-molar limit of detection with a two-step experimental procedure.•Luminescence lifetime monitoring of chemical interference. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0039-9140 1873-3573 |
DOI: | 10.1016/j.talanta.2016.11.017 |