Autophagy limits acute myocardial infarction induced by permanent coronary artery occlusion

• Published in: American journal of physiology. Heart and circulatory physiology Vol. 300; no. 6; pp. H2261 - H2271
• Main Authors: Kanamori, Hiromitsu, Takemura, Genzou, Goto, Kazuko, Maruyama, Rumi, Ono, Koh, Nagao, Kazuya, Tsujimoto, Akiko, Ogino, Atsushi, Takeyama, Toshiaki, Kawaguchi, Tomonori, Watanabe, Takatomo, Kawasaki, Masanori, Fujiwara, Takako, Fujiwara, Hisayoshi, Seishima, Mitsuru, Minatoguchi, Shinya
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.06.2011
• Subjects: Adenosine triphosphatase; Animals; Autophagy; Autophagy - drug effects; Autophagy - physiology; Autophagy-Related Protein 5; Cells, Cultured; Coronary Occlusion - complications; Coronary vessels; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Heart attacks; Lysosomal-Associated Membrane Protein 2 - antagonists & inhibitors; Lysosomal-Associated Membrane Protein 2 - metabolism; Macrolides - pharmacology; Male; Mice; Mice, Inbred C57BL; Mice, Transgenic; Microtubule-Associated Proteins - antagonists & inhibitors; Microtubule-Associated Proteins - genetics; Microtubule-Associated Proteins - metabolism; Models, Animal; Myocardial Infarction - etiology; Myocardial Infarction - metabolism; Myocardial Infarction - physiopathology; Myocardial Ischemia - metabolism; Myocardial Ischemia - physiopathology; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; Myocytes, Cardiac - pathology; RNA, Small Interfering - pharmacology
• ISSN: 0363-6135; 1522-1539
• DOI: 10.1152/ajpheart.01056.2010

Ischemia is known to potently stimulate autophagy in the heart, which may contribute to cardiomyocyte survival. In vitro, transfection with small interfering RNAs targeting Atg5 or Lamp-2 (an autophagy-related gene necessary, respectively, for the initiation and digestion step of autophagy), which specifically inhibited autophagy, diminished survival among cultured cardiomyocytes subjected to anoxia and significantly reduced their ATP content, confirming an autophagy-mediated protective effect against anoxia. We next examined the dynamics of cardiomyocyte autophagy and the effects of manipulating autophagy during acute myocardial infarction in vivo. Myocardial infarction was induced by permanent ligation of the left coronary artery in green fluorescent protein-microtubule-associated protein 1 light chain 3 (GFP-LC3) transgenic mice in which GFP-LC3 aggregates to be visible in the cytoplasm when autophagy is activated. Autophagy was rapidly (within 30 min after coronary ligation) activated in cardiomyocytes, and autophagic activity was particularly strong in salvaged cardiomyocytes bordering the infarcted area. Treatment with bafilomycin A1, an autophagy inhibitor, significantly increased infarct size (31% expansion) 24 h postinfarction. Interestingly, acute infarct size was significantly reduced (23% reduction) in starved mice showing prominent autophagy before infarction. Treatment with bafilomycin A1 reduced postinfarction myocardial ATP content, whereas starvation increased myocardial levels of amino acids and ATP, and the combined effects of bafilomycin A1 and starvation on acute infarct size offset one another. The present findings suggest that autophagy is an innate and potent process that protects cardiomyocytes from ischemic death during acute myocardial infarction.