Molecular Epidemiological Survey and Genetic Characterization of Anaplasma Species in Mongolian Livestock

• Published in: Vector borne and zoonotic diseases (Larchmont, N.Y.) Vol. 17; no. 8; p. 539
• Main Authors: Ochirkhuu, Nyamsuren, Konnai, Satoru, Odbileg, Raadan, Murata, Shiro, Ohashi, Kazuhiko
• Format: Journal Article
• Language: English
• Published: United States 01.08.2017
• Subjects: Anaplasma - genetics; Anaplasma - isolation & purification; Anaplasmosis - epidemiology; Anaplasmosis - microbiology; Animals; Chaperonin 60 - genetics; Chaperonin 60 - metabolism; DNA, Bacterial - genetics; Gene Expression Regulation, Bacterial; Livestock - microbiology; Molecular Epidemiology; Mongolia - epidemiology; Phylogeny; Zoonoses; 16S rRNA; msp4; Mongolia; Anaplasma; Anaplasma ovis; groEL
• ISSN: 1557-7759
• DOI: 10.1089/vbz.2017.2111

Anaplasma species are obligate intracellular rickettsial pathogens that cause great economic loss to the animal industry. Few studies on Anaplasma infections in Mongolian livestock have been conducted. This study examined the prevalence of Anaplasma marginale, Anaplasma ovis, Anaplasma phagocytophilum, and Anaplasma bovis by polymerase chain reaction assay in 928 blood samples collected from native cattle and dairy cattle (Bos taurus), yaks (Bos grunniens), sheep (Ovis aries), and goats (Capra aegagrus hircus) in four provinces of Ulaanbaatar city in Mongolia. We genetically characterized positive samples through sequencing analysis based on the heat-shock protein groEL, major surface protein 4 (msp4), and 16S rRNA genes. Only A. ovis was detected in Mongolian livestock (cattle, yaks, sheep, and goats), with 413 animals (44.5%) positive for groEL and 308 animals (33.2%) positive for msp4 genes. In the phylogenetic tree, we separated A. ovis sequences into two distinct clusters based on the groEL gene. One cluster comprised sequences derived mainly from sheep and goats, which was similar to that in A. ovis isolates from other countries. The other divergent cluster comprised sequences derived from cattle and yaks and appeared to be newly branched from that in previously published single isolates in Mongolian cattle. In addition, the msp4 gene of A. ovis using same and different samples with groEL gene of the pathogen demonstrated that all sequences derived from all animal species, except for three sequences derived from cattle and yak, were clustered together, and were identical or similar to those in isolates from other countries. We used 16S rRNA gene sequences to investigate the genetically divergent A. ovis and identified high homology of 99.3-100%. However, the sequences derived from cattle did not match those derived from sheep and goats. The results of this study on the prevalence and molecular characterization of A. ovis in Mongolian livestock can facilitate the control of infectious diseases in livestock.