Insights on the intestinal absorption of chlorophyll series from microalgae

• Published in: Food research international Vol. 140; p. 110031
• Main Authors: Fernandes, Andrêssa S., Nascimento, Tatiele C., Pinheiro, Pricila N., de Rosso, Veridiana V., de Menezes, Cristiano R., Jacob-Lopes, Eduardo, Zepka, Leila Q.
• Format: Journal Article
• Language: English
• Published: Canada Elsevier Ltd 01.02.2021
• Subjects: Bioaccessibility; Bioactive compounds; Caco-2 cells; Digestive stability; In vitro digestion; Scenedesmus obliquus; Digestive stability; Bioaccessibility; Caco-2 cells; In vitro digestion; Bioactive compounds; Scenedesmus obliquus
• ISSN: 0963-9969; 1873-7145
• DOI: 10.1016/j.foodres.2020.110031

[Display omitted] •Chlorophylls bioaccessibility depends on the form of ingestion.•Chlorophylls in the extract are the most bioaccessible than wet and dried biomass.•Five compounds were bioaccessible, being pheophytin a' in all products.•Hydroxypheophytin a is the chlorophyll derivative most Caco-2 cell absorbable.•It’s the first time to find that pheophytin a' is uptake by Caco-2 cells. The bioaccessibility and subsequent uptake by Caco-2 human intestinal cells of chlorophyll pigments from Scenedesmus obliquus were determined for the first time. In order to evaluate the impact of different types of the matrix on bioaccessibility of chlorophyll from microalgae, three different products were evaluated: isolated chlorophyll extract (ICE); wet ultrasonicated biomass (WUB); and whole dried biomass (WDB). The samples were submitted to in vitro digestion model according to the INFOGEST protocol, and Caco-2 cells determined the intestinal uptake. Chlorophyll pigments were determined by HPLC-PDA-MS/MS. A total of ten chlorophyll pigments (8,318.48 µg g−1) were separated in S. obliquus biomass, with chlorophyll a (3,507.76 µg g−1) and pheophytin a' (1,598.09 µg g−1) the major ones. After in vitro digestion, all tested products showed bioaccessible chlorophylls. However, the total bioaccessibility results were as follows: ICE (33.45%), WUB (2.65%), WDB (0.33%). Five compounds were bioaccessible in ICE, three in WUB, and one in WDB. The hydroxypheophytin a showed the highest bioaccessibility (212%) in ICE, while pheophytin a' in WUB (11%) and WDB (2%). As a result, bioavailability estimates of ICE using the Caco-2 cell showed hydroxypheophytin a (102.53%), followed by pheophytin a' (64.69%) as the chlorophyll pigments most abundant in intestinal cells. In summary, from a nutritional perspective, these three types of the matrix (WDB, WUB, and ICE) influence the promotion of chlorophyll bioaccessibility. In this way, the data suggest that chlorophylls bioaccessibility from ICE is greater than that in WDB and WUB. Therefore, ICE should be considered a product that provides bioavailable chlorophyll and could be the best choice, such as ingredients in the development of functional foods chlorophyll-based.