Antitumor activity of a Rhodococcus sp. Lut0910 isolated from polluted soil

• Published in: Tumor biology Vol. 39; no. 6; p. 1010428317711661
• Main Authors: Zhang, Xin-Guo, Liu, Zi-Yu, Liu, Jin-Wen, Zeng, Yan-Long, Guo, Guang-Jun, Sun, Qiao-Yun
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.06.2017; Sage Publications Ltd
• Subjects: Actinomycetes; Animals; Antitumor activity; Authorship; Cancer therapies; Carcinoma, Hepatocellular - drug therapy; Carcinoma, Hepatocellular - pathology; Cell Extracts - administration & dosage; Cell Extracts - chemistry; Cervical cancer; Cervical carcinoma; Cervix; Cytotoxicity; Deoxyribonucleic acid; DNA; Female; Glucose; Hep G2 Cells; Hepatocellular carcinoma; Humans; Liver Neoplasms - drug therapy; Liver Neoplasms - pathology; Medical research; Metabolites; Mice; Microorganisms; Nucleotide sequence; Oral administration; Pharmaceutical industry; Potassium; Recombinant DNA; Rhodococcus; Rhodococcus - chemistry; RNA, Ribosomal, 16S - genetics; Soil contamination; Soil Pollutants - chemistry; Solid tumors; Spleen; Thermal cycling; Thymus; Tumor cell lines; Uterine Cervical Neoplasms - drug therapy; Uterine Cervical Neoplasms - pathology; Xenograft Model Antitumor Assays; China; polluted soil; Actinomycetes; antitumor activity; Rhodococcus sp
• ISSN: 1010-4283; 1423-0380
• DOI: 10.1177/1010428317711661

The actinomycetes strain, lut0910, was isolated from polluted soil and identified as the Rhodococcus species with 99% similarity based on the sequence analysis of 16S recombinant DNA. The extract of this strain demonstrated in vivo and in vitro antitumor activity. The treatment of two human cancer cell lines, hepatocellular carcinoma HepG2 and cervical carcinoma Hela cells, with the lut0910 extract caused the delay in cell propagation in a dose-dependent manner with an IC50 of 73.39 and 33.09 µg/mL, respectively. Also, the oral administration of lut0910 extract to the mice with a solid tumor resulted in the inhibition of tumor growth in comparison with a placebo group. The thymus and spleen indexes were significantly increased in mice groups treated with the lut0910 extract. The histopathological changes of the tumor tissues showed that there were massive necrotic areas in the tumor tissues after treatment with different doses of the lut0910 extract. Our result would provide a new way and potent source for development of new anticancer agent from the polluted environment.