Effect of Glutathione, Ascorbic Acid and Multivitamins on Sensitivity of Norfloxacin against Pseudomonas aeruginosa and Klebsiella pneumoniae

Several studies have suggested that irrespective of the mode of action of antibiotics, induction of oxidative stress also contributes to their bactericidal actions. When antioxidants are supplemented with antibiotics, the bactericidal effect of the antibiotic are reduced. Thus, in this study, sensit...

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Published inJournal of pure & applied microbiology : an international research journal of microbiology Vol. 13; no. 2; pp. 835 - 840
Main Authors Paulraj, Ponnaiah, Ozogbuda, Prince, Sajna, Keeyari Purayil, Chandramohan, Muruganandham, Ketharin, Tunasamy, Javad, Pazhayakath Thevarkattil Mohamed, Sajeesh, Pattammadath, Natesan, Gopal, P., Raji, A., Jenifer Selvarani
Format Journal Article
LanguageEnglish
Published Oriental Scientific Publishing Company 01.06.2019
Journal of Pure and Applied Microbiology
Subjects
Analysis
Antioxidants
Bacterial pneumonia
klebsiella pneumoniae
multivitamin
Multivitamins
Norfloxacin
Organic acids
oxidative stress
Pneumonia
Pseudomonas aeruginosa
Thiols
Malaysia
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Summary:Several studies have suggested that irrespective of the mode of action of antibiotics, induction of oxidative stress also contributes to their bactericidal actions. When antioxidants are supplemented with antibiotics, the bactericidal effect of the antibiotic are reduced. Thus, in this study, sensitivity of norfloxacin was analysed in the presence of antioxidants like glutathione and ascorbic acids, then the influence of multivitamins in the sensitivity was also analysed. The effect was studied though disc diffusion, MIC (minimal inhibitory concentration) and DNA damage assays against the model organisms - Pseudomonas aeruginosa and Klebsiella pneumoniae. This study suggested that the antioxidant and vitamin solution were having influence over sensitivity towards the antibiotics whose MIC was determined at highest concentration with reduced zone of inhibition and DNA damage. Hence, these results lead to a hypothetical claim for the involvement of multivitamins / antioxidants to have antibacterial resistance development. Keywords: Pseudomonas aeruginosa, Klebsiella pneumoniae, Norfloxacin, Oxidative stress, multivitamin.
ISSN:0973-7510
2581-690X
DOI:10.22207/JPAM.13.2.18