Arsenate and arsenite exposure modulate antioxidants and amino acids in contrasting arsenic accumulating rice (Oryza sativa L.) genotypes

• Published in: Journal of hazardous materials Vol. 262; pp. 1123 - 1131
• Main Authors: Dave, Richa, Tripathi, Rudra Deo, Dwivedi, Sanjay, Tripathi, Preeti, Dixit, Garima, Sharma, Yogesh Kumar, Trivedi, Prabodh Kumar, Corpas, Francisco J., Barroso, Juan B., Chakrabarty, Debasis
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.11.2013
• Subjects: Amino acids; Amino Acids - chemistry; Antioxidants; Antioxidants - chemistry; Arsenates - chemistry; Arsenic - analysis; Arsenic - chemistry; Arsenic accumulation; Arsenites - chemistry; Ascorbate Peroxidases - metabolism; Genotype; Groundwater; Hydroponics; Lipid Peroxidation; Malondialdehyde - chemistry; Metals - chemistry; Oryza - genetics; Oryza - metabolism; Oryza sativa; Oxidative Stress; Peroxidase - metabolism; Plant Roots - metabolism; Reactive Oxygen Species - chemistry; Soil Pollutants - chemistry; Superoxide Dismutase - metabolism; Antioxidants; Oryza sativa; Amino acids; Arsenic accumulation
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2012.06.049

► Contrasting As accumulating rice genotypes were selected from 303 rice cultivars. ► About 13 fold difference was observed between HARG and LARG for As accumulation. ► The induction of antioxidant was more in HARG than LARG along with MDA content. ► Total amino acids positively correlated in LARG and negatively correlated in HARG. ► Stress responsive amino acids showed higher accumulation in HARG than LARG. Carcinogenic arsenic (As) concentrations are found in rice due to irrigation with contaminated groundwater in South-East Asia. The present study evaluates comparative antioxidant property and specific amino acid accumulation in contrasting rice genotypes corresponding to differential As accumulation during arsenate (AsV) and arsenite (AsIII) exposures. The study was conducted on two contrasting As accumulating rice genotypes selected from 303 genotype accessions, in hydroponic conditions. Maximum As accumulation was up to 1181μgg−1 dw in the roots of high As accumulating genotype (HARG), and 89μgg−1 dw in low As accumulating genotype (LARG) under AsIII exposures. The inorganic As was correlated more significantly upon exposures to AsIII than AsV. In the presence of AsV various antioxidant enzymes guiacol peroxidase (GPX), ascorbate peroxidase (APX) and superoxide dismutase (SOD) were highly stimulated in HARG. The stress responsive amino acids proline, cysteine, glycine, glutamic acid and methionine showed higher accumulation in HARG than LARG. A clear correlation was found between stress responsive amino acids, As accumulation and antioxidative response. The comparisons between the contrasting genotypes helped to determine the significance of antioxidants and specific amino acid response to As stress.