Valorization of fruit processing waste to produce high value-added bacterial nanocellulose by a novel strain Komagataeibacter xylinus IITR DKH20

• Published in: Carbohydrate polymers Vol. 260; p. 117807
• Main Authors: Khan, Hina, Saroha, Vaishali, Raghuvanshi, Sharad, Bharti, Amit Kumar, Dutt, Dharm
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.05.2021
• Subjects: Acetobacteraceae - classification; Acetobacteraceae - genetics; Acetobacteraceae - isolation & purification; Acetobacteraceae - metabolism; Bacterial nanocellulose; Cellulose - chemistry; Cellulose - isolation & purification; Cellulose - metabolism; Central composite design; Fruit - microbiology; Fruits peel waste; Komagataeibacter xylinus; Malus - microbiology; Microscopy, Atomic Force; Nanostructures - chemistry; Phylogeny; RNA, Ribosomal, 16S - chemistry; RNA, Ribosomal, 16S - isolation & purification; RNA, Ribosomal, 16S - metabolism; Spectroscopy, Fourier Transform Infrared; Bacterial nanocellulose; Komagataeibacter xylinus; Central composite design; Fruits peel waste
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2021.117807

[Display omitted] •New isolate IITRDKH20 is a high-yield BNC producing bacterium of Komagataeibacter genus.•Komagataeibacter xylinus IITRDKH20 strain has capability to produce 1.23 g L−1 BNC at pH 9.•RSM approach has increased the BNC yield by 4.5 folds.•Valorization of fruits industry wastes into value-added product. To date, the production of bacterial nanocellulose (BNC) by standard methods has been well known, while the use of low-cost feedstock as an alternative medium still needs to be explored for BNC commercialization. This study explores the prospect for the use of the different aqueous extract of fruit peel wastes (aE-FPW) as a nutrient and carbon source for the production of BNC. Herein, this objective was accomplished by the use of a novel, high- yielding strain, isolated from rotten apple and further identified as Komagataeibacter xylinus IITR DKH20 using 16 s rRNA sequencing analysis. The physicochemical properties of BNC matrix collected from the various aE-FPW mediums were similar or advanced to those collected with the HS medium. Statistical optimization of BNC based on Central Composite Design was performed to study the effect of significant parameters and the results demonstrated that the BNC yield (11.44 g L−1) was increased by 4.5 fold after optimization.