Micro-aeration assisted with electrogenic respiration enhanced the microbial catabolism and ammonification of aromatic amines in industrial wastewater

• Published in: Journal of hazardous materials Vol. 448; p. 130943
• Main Authors: Shi, Ke, Cheng, Haoyi, Cornell, Carolyn R., Wu, Haiwei, Gao, Shuhong, Jiang, Jiandong, Liu, Tiejun, Wang, Aijie, Zhou, Jizhong, Liang, Bin
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.04.2023
• Subjects: Amines; Ammonification; Aniline Compounds; Dioxygenases; Electrogenic respiration; Functional genes; Micro-aeration; Microbial associations; Nitrogen Cycle; Respiration; RNA, Ribosomal, 16S; Wastewater; Electrogenic respiration; Micro-aeration; Microbial associations; Ammonification; Functional genes
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2023.130943

Improvement of refractory nitrogen-containing organics biodegradation is crucial to meet discharged nitrogen standards and guarantee aquatic ecology safety. Although electrostimulation accelerates organic nitrogen pollutants amination, it remains uncertain how to strengthen ammonification of the amination products. This study demonstrated that ammonification was remarkably facilitated under micro-aerobic conditions through the degradation of aniline, an amination product of nitrobenzene, using an electrogenic respiration system. The microbial catabolism and ammonification were significantly enhanced by exposing the bioanode to air. Based on 16S rRNA gene sequencing and GeoChip analysis, our results indicated that aerobic aniline degraders and electroactive bacteria were enriched in suspension and inner electrode biofilm, respectively. The suspension community had a significantly higher relative abundance of catechol dioxygenase genes contributing to aerobic aniline biodegradation and reactive oxygen species (ROS) scavenger genes to protect from oxygen toxicity. The inner biofilm community contained obviously higher cytochrome c genes responsible for extracellular electron transfer. Additionally, network analysis indicated the aniline degraders were positively associated with electroactive bacteria and could be the potential hosts for genes encoding for dioxygenase and cytochrome, respectively. This study provides a feasible strategy to enhance nitrogen-containing organics ammonification and offers new insights into the microbial interaction mechanisms of micro-aeration assisted with electrogenic respiration. [Display omitted] •Aniline (AN) could be degraded under micro-aerobic bioanode system.•Microbial functional differentiation occurred in suspension and electrode.•Functional genes were more sensitive than 16S rRNA genes to DO stimulation.•AN degraders in suspension positively correlated with electroactive bacteria.•AN degraders were potential hosts of dioxygenase catalyzing ammonification.