Characterization and taxonomic placement of Rhizoctonia-like endophytes from orchid roots

• Published in: Mycologia Vol. 94; no. 2; pp. 230 - 239
• Main Authors: Shan, X. C., Liew, E. C. Y., Weatherhead, M. A., Hodgkiss, I. J.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.03.2002; Mycological Society of America
• Subjects: arundina chinensis; Biological taxonomies; Ceratorhiza; cleaved amphlified polymorphic sequences; Ecology; endophytes; Endosymbionts; Epulorhiza; eulophia flava; fungal anatomy; Fungi; Germination; goodyera procera; habenaria dentata; host plants; internal transcribed spacers; molecular sequences data; Mycology; mycorrhiza; mycorrhizal fungi; nucleotide sequences; orchid; Orchidaceae; ornamental plants; phylogeny; plant taxonomy; Polymerase chain reaction; pore ultrastructure; random amplified polymorphic DNA technique; Rhizoctonia; Ribosomal DNA; spathoglottis pubescens; spiranthes hongkongenis; spiranthes hongkongensis; symbiont; Symbionts; Symbiosis; China
• ISSN: 0027-5514; 1557-2536
• DOI: 10.1080/15572536.2003.11833228

Twenty-one Rhizoctonia-like fungal strains were isolated from the roots of four terrestrial orchid species from various locations in Hong Kong. The cultural morphology, nuclear number of the hyphal cell, pore ultrastructure, and RAPD and CAPS analyses of rDNA fragments revealed that most of these isolates were associated with the genera Ceratorhiza and Epulorhiza. RAPD analysis showed the presence of genetic diversity between the isolates from different hosts and locations. The compatibility between a selection of these Ceratorhiza and Epulorhiza isolates and 14 orchid species was determined using a symbiotic germination method. The germination and development of three orchid species, Arundina chinensis, Spathoglottis pubescens, and Spiranthes hongkongensis, were strongly stimulated by the Epulorhiza isolates. Habenaria dentata was found to form symbionts successfully with a Ceratorhiza isolate.