Development of a high-performance liquid chromatography method for the determination of florfenicol in animal feedstuffs

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1068-1069; pp. 9 - 14
• Main Authors: Yang, JinJing, Sun, GuiZhi, Qian, MingRong, Huang, LingLi, Ke, XianBing, Yang, Bo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.11.2017
• Subjects: Animal Feed - analysis; Animal feedstuffs; Chromatography, High Pressure Liquid - methods; Chromatography, Thin Layer - methods; Florfenicol; High-performance liquid chromatography; Limit of Detection; Linear Models; Reproducibility of Results; Thiamphenicol - analogs & derivatives; Thiamphenicol - analysis; Thiamphenicol - isolation & purification; Thin layer chromatography; FF; LOD; UV; RSD; LC–MS; Animal feedstuffs; SPE; TLC; High-performance liquid chromatography; LOQ; Thin layer chromatography; Florfenicol; HPLC
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2017.09.045

•A TLC-HPLC method was developed for determining florfenicol in animal feedstuffs.•The analytical method was proved to be reliable, simple and cost-effective.•TLC could be used as an alternative purification technique for complex matrix. An effective thin layer chromatography (TLC) purification procedure coupled to high-performance liquid chromatography (HPLC) method was developed for the determination of florfenicol (FF) in pig, chicken and fish feedstuffs. The feedstuff samples were extracted with ethyl acetate, defatted with n-hexane saturated with acetonitrile, and further purified by TLC. The chromatographic separation was performed on a Waters Symmetry C18 column using an isocratic procedure with acetonitrile-water (35:65, v/v) at 0.6mL/min. The ultraviolet (UV) detector was set at a wavelength of 225nm. The FF concentrations in feedstuff samples were quantified using a standard curve. Good linear correlations (y=159075x−15054, r>0.9999) were achieved within the concentration range of 0.05–200μg/mL. The recoveries of FF spiked at levels of 1, 100 and 1000μg/g ranged from 80.6% to 105.3% with the intra-day and inter-day relative standard deviation (RSD) less than 9.3%. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.02 and 0.06mg/kg for pig feedstuffs, 0.02 and 0.07mg/kg for chicken feedstuffs, and 0.02 and 0.05mg/kg for fish feedstuffs, respectively. This reliable, simple and cost-effective method could be applied to the routine monitoring of FF in animal feedstuffs.