Optimization of β-Glucuronidase Activity from Lactobacillus delbrueckii Rh2 and Its Use for Biotransformation of Baicalin and Wogonoside

• Published in: Applied biological chemistry Vol. 54; no. 2; pp. 275 - 280
• Main Authors: Ku, S.M., Seoul National University, Seoul, Republic of Korea, Zheng, Hua, Seoul National University, Seoul, Republic of Korea, Park, M.S., Anyang Science University, Anyang, Republic of Korea, Ji, G.E., Seoul National University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.04.2011; Springer Nature B.V; 한국응용생명화학회
• Subjects: Aglycones; Applied Microbiology; Baicalin; beta-glucuronidase; Biological Techniques; Bioorganic Chemistry; Biotransformation; Chemistry; Chemistry and Materials Science; Enzymatic activity; Enzyme activity; Enzymes; Flavonoids; Food Science/Microbiology; Galactose; Herbal medicine; Intestinal microflora; Intestine; Lactobacilli; LACTOBACILLUS; Lactobacillus delbrueckii; Medicinal plants; Optimization; scutellariae radix; wogonoside; 농학; β-glucuronidase; wogonoside; baicalin; scutellariae radix
• ISSN: 1738-2203; 2468-0834; 2234-344X; 2468-0842
• DOI: 10.3839/jksabc.2011.043

Scutellariae radix, the root of Scutellaria baicalensis, is a traditional medicinal herb containing baicalin and wogonoside as the main flavonoids. Before absorption, these flavonoids are metabolized into their corresponding aglycones, baicalein and wogonin, by β-glucuronidase produced by the intestinal microflora. To develop a method for efficient transformation of these flavonoids, optimal conditions for the production of β-glucuronidase from Lactobacillus delbrueckii Rh2 (Rh2) were determined. Addition of 4% galactose to basal medium increased the enzyme activity of Rh2 by about 8-fold. Optimal pH and temperature for the β-glucuronidase activity of cell extract were 5.0 and 50℃, respectively, and more than 95% of the enzyme activity was maintained at 60℃ for 11 h. Under optimal conditions, more than 90% of the glycones were converted into their corresponding aglycones within 3 h. These results demonstrate that the transformation of baicalin and wogonoside from S. baicalensis by Rh2 would be useful for the efficient production of wogonin and baicalein.