Survival of the nematophagous fungus Duddingtonia flagrans to in vitro segments of sheep gastrointestinal tract

• Published in: Experimental parasitology Vol. 231; p. 108172
• Main Authors: Céspedes-Gutiérrez, E., Aragón, D.M., Gómez-Álvarez, M.I., Cubides-Cárdenas, J.A., Cortés-Rojas, D.F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2021
• Subjects: Abomasum - microbiology; Abomasum - parasitology; Analysis of Variance; Animals; Ascomycota - growth & development; Ascomycota - physiology; Biological control; Duddingtonia flagrans; Feces - parasitology; Gastrointestinal Diseases - microbiology; Gastrointestinal Diseases - parasitology; Gastrointestinal Diseases - prevention & control; Gastrointestinal nematodes; Gastrointestinal Tract - microbiology; Gastrointestinal Tract - parasitology; In vitro simulation; Intestine, Small - microbiology; Intestine, Small - parasitology; Mouth - microbiology; Mouth - parasitology; Nematode Infections - microbiology; Nematode Infections - prevention & control; Nematophagous fungi; Pest Control, Biological - methods; Rumen - microbiology; Rumen - parasitology; Sheep; Spores, Fungal - growth & development; Duddingtonia flagrans; Nematophagous fungi; Sheep; Gastrointestinal nematodes; In vitro simulation; Biological control
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/j.exppara.2021.108172

The nematophagous fungus Duddingtonia flagrans is used in integrated management of gastrointestinal nematodes in ruminants. The chlamydospores of the fungus, orally administered, pass through the segments of the ruminant digestive tract and, in the feces, capture the nematodes preventing their migration to grasslands. The drastic conditions of the gastrointestinal segments can negatively affect the fungus' biocontrol activity. The aim of this study was to assess the effect of in vitro conditions of the sheep's main gastrointestinal segments on the concentration, viability and nematode predatory ability of D. flagrans chlamydospores. The segments evaluated separately in vitro were the oral cavity, rumen, abomasum, and small intestine. The results showed that chlamydospores concentration was not affected by exposure to the different segments. The viability of the chlamydospores after exposure to the oral cavity (2.53 × 106 CFU/mL) and small intestine (1.24 × 105 CFU/mL) was significantly lower than its control treatment, with values of 6.67 × 106 CFU/mL and 2.31 × 105 CFU/mL respectively. Nematode predatory ability after rumen exposure was reduced by 7% compared to the control treatment, by 25% after abomasum exposure and by 17% after small intestine. This study revealed the individual in vitro effect of each segment of ovine gastrointestinal tract on the integrity of this strain of the fungus D. flagrans affecting its viability and nematode predatory ability under the evaluated conditions. Delivery systems could be designed to protect chlamydospores considering the impact of each gastrointestinal segment. [Display omitted] •In vitro conditions of oral cavity and small intestine affected D. flagrans viability.•Exposure to in vitro abomasum reduce fungus nematode predatory ability.•Predatory ability might be affected even when concentration and viability were not.