Visible light-curable methacrylated glycol chitosan hydrogel patches for prenatal closure of fetal myelomeningocele

• Published in: Carbohydrate polymers Vol. 311; p. 120620
• Main Authors: Ko, Hyun Sun, Yang, Dae Hyeok, Kim, Ahyoung, Wie, Jeong Ha, Kim, Sae Hyun, Jeong, Gun-Jae, Hyun, Hoon, Shin, Jong Chul, Chun, Heung Jae
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.07.2023
• Subjects: Animals; Chitosan - chemistry; Female; Fetal myelomeningocele; Fetal surgical technique; Hydrogels - chemistry; Light; Meningomyelocele - chemically induced; Methacrylated glycol chitosan; Pregnancy; Prenatal treatment; Rats; Visible light curable hydrogel patch; Fetal surgical technique; Methacrylated glycol chitosan; Prenatal treatment; Visible light curable hydrogel patch; Fetal myelomeningocele
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2023.120620

In this study, we prepared visible light-curable methacrylated glycol chitosan (MGC) hydrogel patches for the prenatal treatment of fetal myelomeningocele (MMC) and investigated their feasibility using a retinoic acid-induced fetal MMC rat model. 4, 5, and 6 w/v% of MGC were selected as candidate precursor solutions, and photo-cured for 20 s, because the resulting hydrogels were found to possess concentration dependent tunable mechanical properties and structural morphologies. Moreover, these materials exhibited no foreign body reactions with good adhesive properties in animal studies. The inflammation scoring assessment in vivo exhibited the absence of foreign body reactions in MGC hydrogel treated lesion. The complete epithelial coverage of MMC was made with using 6 w/v% MGC hydrogel followed by well-organized granulation along with noticeable decrease of abortion rate and wound size that highlight the therapeutic potential for the prenatal treatment of fetal MMC. [Display omitted]