Poly (ADP-ribose) polymerase-1 (PARP-1) in Chinese patients with Graves’ disease and Graves’ ophthalmopathy

• Published in: Canadian journal of physiology and pharmacology Vol. 96; no. 6; pp. 556 - 561
• Main Authors: Wu, Tong, Tang, Dong-run, Zhao, Liang, Sun, Feng-yuan
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.06.2018; Canadian Science Publishing NRC Research Press
• Subjects: Adolescent; Adult; Aged; Asian Continental Ancestry Group - genetics; Autoimmune diseases; cytokine; Cytokines; Enzyme-linked immunosorbent assay; Expressed sequence tags; Female; gene; Gene polymorphism; Genetic diversity; genetic polymorphism; Genotype; Graves Ophthalmopathy - enzymology; Graves Ophthalmopathy - genetics; Graves' disease; Graves’ ophthalmopathy; Humans; Hyperthyroidism; maladie de Graves; Male; Middle Aged; ophtalmopathie de Graves; Pharmacology; Physiology; poly (ADP-ribose) polymerase-1; Poly (ADP-Ribose) Polymerase-1 - genetics; poly (ADP-ribose) polymérase-1; Poly(ADP-ribose) polymerase; Polymerase chain reaction; Polymorphism; Polymorphism, Genetic; polymorphisme; polymorphisme génétique; Restriction fragment length polymorphism; Young Adult; cytokine; polymorphisme génétique; poly (ADP-ribose) polymérase-1; Graves’ disease; gene; Graves’ ophthalmopathy; maladie de Graves; polymorphism; ophtalmopathie de Graves; poly (ADP-ribose) polymerase-1; genetic polymorphism; polymorphisme
• ISSN: 0008-4212; 1205-7541
• DOI: 10.1139/cjpp-2016-0332

We aimed to evaluate the genetic variation of poly (ADP-ribose) polymerase-1 (PARP-1) as risk factor in development of Graves’ disease (GD) and Graves’ ophthalmopathy (GO) among Chinese individuals. Patients with confirmed diagnosis of GD or healthy individuals with no clinical symptoms of hyperthyroiditis were enrolled at the Department of Ophthalmology, Tianjin First Center Hospital, China. Genetic polymorphism was studied in plasma DNA samples of subjects by polymerase chain reaction of restriction fragment length polymorphism to confirm our hypothesis. Cytokine levels were measured routinely on serum samples of subjects by sandwich ELISA technique. Patients with GG genotype (odds ratio (OR) 95% CI = 2.25 (1.35–3.73), p = 0.002) and carriers of G allele (OR = 2.03 (1.23–3.36), p = 0.006) were at high risk of developing ophthalmopathy. Polymorphism of del/ins of nuclear factor-κB1 gene (NFkB1) gene (OR = 7.1 (2.88–17.52), p < 0.0001) and PARP-1 C410T polymorphism was found to be associated with GO (p < 0.05). Cytokine level was significantly higher in patients with GD (p < 0.05), but no significant change in cytokines level among GO patients from baseline (p > 0.05). Our study results recommended that polymorphism of PARP-1 gene is more likely responsible for development of GD in Chinese individuals. We also observed that the polymorphism of gene-related del/ins to NFkB1 in development of GO.