Additional calcium and sulfur manages hexavalent chromium toxicity in Solanum lycopersicum L. and Solanum melongena L. seedlings by involving nitric oxide

• Published in: Journal of hazardous materials Vol. 398; p. 122607
• Main Authors: Singh, Samiksha, Mohan Prasad, Sheo, Pratap Singh, Vijay
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.11.2020
• Subjects: Calcium; Chromium (VI) toxicity; Chromium - toxicity; Lycopersicon esculentum; Nitric Oxide; Oxidative Stress; Phytochelatins; Seedlings; Solanum melongena; Sulfur; Tolerance; Vacuolar sequestration; Vegetables; Tolerance; Phytochelatins; Vegetables; Chromium (VI) toxicity; Vacuolar sequestration
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2020.122607

[Display omitted] •Cr(VI) altered root traits and photosynthesis and hence reduced growth.•Cr(VI significantly accumulated into cell organelles.•Addition of Ca and S mitigated Cr(VI) toxicity by reducing Cr(VI) uptake in cells.•Addition of Ca and S promoted sequestration of Cr into vacuoles.•Ca and S-mediated management of Cr(VI) toxicity involves nitric oxide. In recent years, nutrient management has gained much attention for mitigating metal stress. But, role of nutrients like calcium (Ca) and sulfur (S) in mitigating Cr(VI) toxicity along with their mechanism of action are still limited. Therefore, the present study was performed to explore role of Ca and S in ameliorating Cr(VI) toxicity in 21 days old seedlings of Solanum lycopersicum L. and Solanum melongena L. Chromium (VI) reduced tolerance index and altered root traits due to greater Cr accumulation in the cell wall and cellular organelles due to down-regulation in thiols and phytochelatins that lead to alterations in photosynthesis. However, Ca or S stimulated vacuolar sequestration of Cr(VI) and reduced its uptake at the cell wall. This was coincided with up-regulation in glutathione-S-transferase activity, and amounts of thiols and phytochelatins. Cr(VI) caused oxidative stress together with up-regulation in superoxide dismutase and catalase, and proline metabolism while Ca and S reversed these effects. Chromium (VI) inhibited nitrate reductase activity while Ca and S reversed this response. NG-nitro-l-arginine methyl ester augmented Cr(VI) toxicity but sodium nitroprusside (SNP) mitigated Cr(VI) toxicity. Overall results show that Ca and S both are able in ameliorating Cr(VI) toxicity and require nitric oxide for this task.