Establishing a Assay for Detection of Nonylphenol Estrogenic Effects

Environmental estrogen could mimic natural estrogens thereby disrupting the endocrine systems of human and animals. The actions of such endocrine disruptors have been studied mainly on reproduction and development. To explore the estrogenic effects of NP by reporter genebased assays we developed. pE...

Full description

Saved in:
Bibliographic Details
Published inApplied Mechanics and Materials Vol. 71-78; pp. 3003 - 3006
Main Authors Lu, Xiong Bing, Liu, Yan Qun
Format Journal Article
LanguageEnglish
Published Zurich Trans Tech Publications Ltd 01.07.2011
Subjects
Estrogenic Activity
SPC-A1 Cells
Nonylphenol
Online AccessGet full text

Cover

More Information
Summary:Environmental estrogen could mimic natural estrogens thereby disrupting the endocrine systems of human and animals. The actions of such endocrine disruptors have been studied mainly on reproduction and development. To explore the estrogenic effects of NP by reporter genebased assays we developed. pERE-GFP plamid was generated by inserting estrogen response element fragment into pGADD153-GFP. the recombinant was confirmed by restriction enzyme map and transfected into SPC-A1 cells to ensure the expression of green fluorescent protein．The assay we established in usful, NP could induce the estrogenic activities at any of the tested concentrations.
Bibliography:Selected, peer reviewed papers from the 2011 International Conference on Green Building, Materials and Civil Engineering, (GBMCE 2011), Shangri-La, China, August 22-23, 2011
ISBN:9783037852033
3037852038
ISSN:1660-9336
1662-7482
1662-7482
DOI:10.4028/www.scientific.net/AMM.71-78.3003