Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure
•PSG-1 was re-purified and the structure was re-elucidated with more evidences.•β-(1→3)-glucan was identified as the backbone with branch point at O-6 of glucose.•Long β-(1→6)-glucose side chain was proposed.•β-(1→4)-GlcA was found in the side chain of PSG-1. The fine structure in terms of backbone...
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Published in | Carbohydrate polymers Vol. 158; pp. 58 - 67 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
20.02.2017
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Subjects | |
Online Access | Get full text |
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Summary: | •PSG-1 was re-purified and the structure was re-elucidated with more evidences.•β-(1→3)-glucan was identified as the backbone with branch point at O-6 of glucose.•Long β-(1→6)-glucose side chain was proposed.•β-(1→4)-GlcA was found in the side chain of PSG-1.
The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC–MS analysis, and 1D & 2D NMR spectroscopy. Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%). Based on methylation, multistep partial acid hydrolysis and NMR study, ▪ were proposed to substitute at the O-6 position of β-(1→3)-glucan. The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2. This revised structure as an acidic β-(1→3, 1→6)-glucan is considered to be more accurate than the previous proposal of PSG-1. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0144-8617 1879-1344 |
DOI: | 10.1016/j.carbpol.2016.11.088 |