SCREENING OF E- AND Z-GUGGULSTERONES IN THE GUM-RESIN EXUDATES OF SOME COMMON PLANTS AND METHOD VALIDATION IN RAW, EXTRACTED, AND PHARMACEUTICAL FORMULATIONS OF COMMIPHORA MUKUL BY HPLC

• Published in: Journal of liquid chromatography & related technologies Vol. 34; no. 18; pp. 2103 - 2117
• Main Authors: Musharraf, Syed Ghulam, Iqbal, Naveed, Ahmed, Muhammad Arif, Mazhar, Shumiala, Choudhary, Muhammad Iqbal
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 01.11.2011; Taylor & Francis; Taylor & Francis Ltd
• Subjects: Abies balsamea and Commiphora stocksiana; Acacia nilotica; Analysis; Biological and medical sciences; Chemical constitution; Commiphora mukul; Delonix regia; E- and Z-guggulsterones; Ficus religiosa; Fundamental and applied biological sciences. Psychology; General pharmacology; guggulipid; guggulu; HPLC analysis; Mangifera indica; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant physiology and development; Asia; China; India; Steroid; Stereoisomer; Chemical analysis; Exudate; Abies balsamea and Commiphora stocksiana; HPLC chromatography; Mango; Biological marker; Commiphora mukul; Medicinal plant; Folk medicine; guggulu; Dicotyledones; Angiospermae; Gymnospermae; Ayurvedic medicine; Diode array; Tablet; Gum; Anacardiaceae; Quantitative analysis; Pregnane derivatives; Validation; Abies balsamea; Fruit; HPLC analysis; Pharmacognosy; E- and Z-guggulsterones; Mangifera indica; Oral form; Moraceae; Burseraceae; Ficus religiosa; Delonix regia; Ultraviolet detector; Leguminosae; Ficus; Dosage form; Coniferales; Spermatophyta; Acacia nilotica; guggulipid
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1080/10826076.2011.585481

Hypolipidemic agents, E- and Z-guggulsterones, are the biomarker compounds found in the gum-resin exudates of Commiphora mukul. Gum-Resin exudates of six common plants included Mangifera indica, Ficus religiosa, Delonix regia, Acacia nilotica, Abies balsamea, and Commiphora stocksiana and were screened against the said drug by the HPLC method. The developed method was validated for the quantification of E- and Z-guggulsterones in the gum-resin exudates of Commiphora mukul (raw form), guggulipid (ethyl acetate extract of gum-resin,) and its pharmaceutical products (finished form) that are the various components involved in the preparation of pharmaceutical products, from raw materials to finished products. The method involved a simple extraction procedure under sonication. The new method was validated as per the ICH recommended guidelines, including specificity, linearity, precision, accuracy, limit of quantitation, and robustness. The method has a validated quantitation range of 2-65 µg/mL with a precision of ±2% SD, recovery of >99.5% and correlation coefficients >0.999 for both E- and Z-guggulsterones. The statistical analysis proves that the method is repeatable and selective for the estimation of the said drugs and can be used as a routine analytical method and applicable to the different processing components involved in the pharmaceutical preparation from Commiphora mukul resin. Supplemental materials are available for this article. Go to the publisher's online edition of the Journal of Liquid Chromatograhy & Related Technologies to view the free supplemental file.