Forced degradation of l-(+)-bornesitol, a bioactive marker of Hancornia speciosa: Development and validation of stability indicating UHPLC-MS method and effect of degraded products on ACE inhibition

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1093-1094; pp. 31 - 38
• Main Authors: Gomes, José Hugo de Sousa, da Silva, Grazielle Caroline, Côrtes, Steyner F., de Pádua, Rodrigo Maia, Braga, Fernão Castro
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2018
• Subjects: ACE inhibition; Forced degradation; l-(+)-Bornesitol; LC-MS analysis; MRM; myo-Inositol; MRM; LC-MS analysis; Forced degradation; ACE inhibition; myo-Inositol; l-(+)-Bornesitol
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2018.06.045

The antihypertensive activity of the medicinal plant Hancornia speciosa has been previously demonstrated by us, being the activity ascribed to polyphenols and cyclitols like l-(+)-bornesitol. We herein evaluated the stability of the bioactive marker bornesitol submitted to forced degradation conditions. Bornesitol employed in the study was isolated from H. speciosa leaves. An UHPLC-ESI-MS/MS method was developed to investigate bornesitol stability based on MRM (Multiple Reaction Monitoring) acquisition mode and negative ionization mode, employing both specific (m/z 193 → 161 Da) and confirmatory (m/z 193 → 175 Da) transitions. A gradient elution of 0.1% formic acid in water and acetonitrile was performed on a HILIC column. The method was validated and showed adequate linearity (r2 > 0.99), selectivity, specificity, accuracy, and precision (RSD < 2.9%). The method was robust for deliberate variations on dessolvation temperature, but not for changes in the flow rate and dessolvation gas. The results from the stability studies allowed us to classify bornesitol as labile for acidic and alkaline hydrolysis, but as very stable for oxidative and neutral hydrolysis exposure. Bornesitol was categorized as practically stable under photolysis degradation, whereas a considerable reduction on its contents was induced by metal ions and thermolysis exposure. Degraded samples from neutral hydrolysis and thermolysis were assayed in vitro for ACE inhibition and showed a substantial decrease in biological activity as compared to intact bornesitol. myo-Inositol was identified as the major degradation products in both matrices. This is the first report on bornesitol stability under different stress conditions and the obtained data are relevant for the development and quality control of standardized products from H. speciosa leaves. •l-(+)-Bornesitol was isolated from Hancornia speciosa leaves.•An UHPLC-MS method was developed to quantify bornesitol after forced degradation.•MS method employed MRM acquisition mode with specific and confirmatory transitions.•myo-Inositol was found as the main degradation product from neutral hydrolysis and thermolysis.•Degradation of bornesitol led to reduction in ACE inhibitory activity.