Definitely simultaneous determination of three lignans in rat using ultra-high performance liquid chromatography-tandem mass spectrometry

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1100-1101; pp. 17 - 26
• Main Authors: Li, Yueting, Sun, Jing, Huo, Huixia, Liu, Yao, Liu, Wenjing, Zhang, Qian, Zhao, Yunfang, Song, Yuelin, Li, Jun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.11.2018
• Subjects: Animals; Chromatography, High Pressure Liquid - methods; Drug Stability; Fruits of Vitex negundo var. cannabifolia; Identity consolidation; Lignan; Lignans - blood; Lignans - pharmacokinetics; Limit of Detection; Linear Models; Male; MS2 spectrum; Pharmacokinetics; Plant Extracts - administration & dosage; Plant Extracts - pharmacokinetics; Rats; Rats, Sprague-Dawley; Relative response-collision energy curve; Reproducibility of Results; Tandem Mass Spectrometry - methods; Lignan; Relative response-collision energy curve; MS2 spectrum; Fruits of Vitex negundo var. cannabifolia; Pharmacokinetics; Identity consolidation; MS spectrum
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2018.09.024

Herbal medicines (HMs) have been widely demonstrated to be extremely complicated chemical pools, notably the occurrences of isomers that usually possess similar chromatographic and spectrometric behaviors, and the complexity will be further boosted in HMs-treated biological samples. Hence, there is a technical barrier regarding identity confirmation towards the acquisition of reliable quantitative information for those compounds-of-interest in biological matrices, although tandem mass spectrometry (MS/MS) is a favored tool because of the advantages in terms of specificity and selectivity, in particular being hyphenated with ultra-high performance liquid chromatography (UHPLC). More structural information should be involved in addition to retention times and precursor-to-product ion transitions, to consolidate the identities of the captured signals. Attempts were made here to strengthen quantitation confidences via matching MS2 spectra and relative response-collision energy curves (RRCECs) between each pair of suspect peak and the authentic signal, and UHPLC coupled to hybrid triple quadrupole-linear ion trap mass spectrometry (Qtrap-MS) that enabled simultaneous acquisition of qualitative and quantitative information acted as the analytical tool. Definitely simultaneous determination of a pair of regio-isomers namely 6‑hydroxy‑4‑(4‑hydroxy‑3‑methoxyphenyl)‑3‑hydroxymethyl‑7‑methoxy‑3,4‑dihydro‑2‑naphthaldehyde (VB-1) and vitedoin A (VB-2), together with another analogue namely detetrahydroconidendrin (VB-3), in rat plasma as well as tissues was conducted following oral administration of the extract of fruits of Vitex negundo var. cannabifolia. Diverse method validation assays in regard of selectivity, linearity, intra- and inter-day variations, matrix effect, and recovery were employed to demonstrate the newly developed method being able to fulfill the demands of rational quantitation. Overall, the current study offers a promising approach to accomplish confidence-enhanced quantitation in biological matrices. •A novel UHPLC-MS/MS method of 3 lignans in rat plasma and tissues was developed and validated.•MS2 spectra and RRCEC matching was employed to enhance identity confidence.•Three lignans including 2 isomers were determined in DFV-treated plasma and tissues.•The findings should be useful for clarifications of effective material basis of DFV.