Seroconversion of a Thermostable live Attenuated Lentogenic Strain Newcastle Disease Vaccine (Local isolate) in Chicken

• Published in: Journal of pure & applied microbiology : an international research journal of microbiology Vol. 11; no. 2; pp. 981 - 986
• Main Authors: Sharma, L, Biswas, U, Guha, C, Chatterjee, A, Jana, P.S, Pandey, R
• Format: Journal Article
• Language: English
• Published: Oriental Scientific Publishing Company 01.06.2017; Journal of Pure and Applied Microbiology
• Subjects: Care and treatment; chicken; Immunization; Methods; newcastle; Newcastle disease; Poultry vaccines; serum sample; Testing; vaccine; India
• ISSN: 0973-7510; 2581-690X
• DOI: 10.22207/JPAM.11.2.40

Vaccine was prepared by using the isolate, exposed to 43[degrees]C for 12 days. The vaccination was performed in two thousand four hundred breeder flocks (Banoraja) in 3 different flocks @[10.sup.6.5] [EID.sub.50] per bird oronasally at the age of day 5 (primary vaccination) followed by vaccination through drinking water on day 26 (boostering) in farm condition and thereafter every 45 days interval. The vaccine was also inoculated intranasally @[10.sup.6.5] [EID.sub.50] in Two thousand backyard birds (Haringhata Black and non-descriptive) and were boostered after 21 days of 1st vaccination with the same dose and subsequent vaccination was performed at every 45 days interval. Serum samples were collected for HI titre at regular intervals and it was observed that the mean antibody both in backyard birds and breeder flocks was above 1.50 [+ or -] 00. Therefore, it was concluded that the thermostable live attenuated lentogenic strain Newcastle Disease vaccine after inoculation into backyard birds and breeder flocks, provides satisfactory level of immunity. Keywords: Vaccine, Chicken, Newcastle, Serum sample.