Antibacterial activity of compounds isolated from Caesalpinia coriaria (Jacq) Willd against important bacteria in public health

• Published in: Microbial pathogenesis Vol. 136; p. 103660
• Main Authors: Olmedo-Juárez, Agustín, Briones-Robles, Tania Isabel, Zaragoza-Bastida, Adrian, Zamilpa, Alejando, Ojeda-Ramírez, Deyanira, Mendoza de Gives, Pedro, Olivares-Pérez, Jaime, Rivero-Perez, Nallely
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.11.2019
• Subjects: Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Caesalpinia - chemistry; Caesalpinia coriaria (Jacq) Willd; Chemical Fractionation; Chromatography, High Pressure Liquid; Gallic acid; Gallic Acid - analogs & derivatives; Gallic Acid - isolation & purification; Gallic Acid - pharmacology; Gram-Negative Bacteria - drug effects; Gram-Positive Bacteria - drug effects; Magnetic Resonance Spectroscopy; Methyl gallate; Microbial Sensitivity Tests; Microbial Viability - drug effects; Phytochemicals - isolation & purification; Phytochemicals - pharmacology; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Methyl gallate; Antibacterial activity; Caesalpinia coriaria (Jacq) Willd; Gallic acid
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2019.103660

Antimicrobial resistance has been increasing in recent years and is most frequently found in pathogenic microorganisms resistant or multiresistant to drugs. The secondary metabolites of plants have been evaluated as alternatives for control and treatment of these microorganisms. The aim of this study was to isolate and identify the secondary metabolites with antibacterial activity from Caesalpinia coriaria (Jacq) Willd fruit. Hydroalcoholic extract (CCHA), was subjected to a bipartition with ethyl acetate giving two fractions an aqueous (Aq-F) and an organic (EtOAc-F). The isolation of bioactive fraction (EtOAc-F) allowed obtain two important compounds, methyl gallate (1) and gallic acid (2). These compounds were identified by high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR). The CCHA, both fractions and the isolated compounds were evaluated in vitro to determine their Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) against Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Listeria monocytogenes and Staphylococcus aureus. Gallic acid (2) showed the lowest MIC on S. typhi, (0.156 mg/mL), L. monocytogenes and S. aureus (1.25 mg/mL), while methyl gallate (1) had the best inhibitory effect against E. coli and P. aeruginosa (1.25 mg/mL). On the other hand, methyl gallate (1) showed the best MBC on P. aeruginosa (2.50 mg/mL), and gallic acid (2) had the lowest MBC on P. aeruginosa and L. monocytogenes. In conclusion, methyl gallate (1) and gallic acid (2) are the compounds responsible for the antibacterial activity of Caesalpinia coriaria fruit. [Display omitted] •Hidroalcoholic extract from Caesalpinia coriaria fruits has antibacterial activity.•Organic fraction from Caesalpinia coriaria extract is most active fraction.•Methyl gallate and Gallic acid are secondary metabolites from Caesalpinia coriaria.•Methyl gallate and Gallic acid from Caesalpinia coriaria have antibacterial activity.