Effect of asparagine, corncob biochar and Fe(II) on anaerobic biological treatment under low temperature: Enhanced performance and microbial community dynamic

• Published in: Journal of environmental management Vol. 317; p. 115348
• Main Authors: Li, Xinjuan, Qin, Ronghua, Yang, Wenjing, Su, Chengyuan, Luo, Zehua, Zhou, Yijie, Lin, Xiangfeng, Lu, Yingqi
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.2022
• Subjects: Anaerobic enhancement; Asparagine; Biochar; Fe2; Low temperature; Asparagine; Fe2; Anaerobic enhancement; Biochar; Low temperature; Fe(2+)
• ISSN: 0301-4797; 1095-8630
• DOI: 10.1016/j.jenvman.2022.115348

To ensure the efficiency of anaerobic biological treatment technology at lower temperature will expand the application of anaerobic reactor in practical industrial wastewater treatment. Through a batch experiment, asparagine, corncob biochar and Fe2+ were selected as strengthening measures to analyze the effects on the anaerobic sludge characteristics, microbial community and functional genes in the low temperature (15 °C). Results showed that after 21 days, asparagine began to promote chemical oxygen demand (COD) removal by the anaerobic treatment, with highest COD removal rate (81.65%) observed when the asparagine concentration was 1 mmol/L. When adding 3 g biochar, 25 mg/L Fe2+, and the combination of biochar and Fe2+, the COD removal rates reached to 82%, 92% and 97%, respectively. In the presence of asparagine, both biochar and Fe2+ alone or in combination increased the activity of protease (16.35%–120.71%) and coenzyme F420 (5.63%–130.2%). The relative abundance of Proteobacteria and Methanobacterium increased in the presence of biochar and Fe2+. In addition, the KEGG results showed that the combined addition of biochar and Fe2+ enhanced bacterial replication and repair and promoted amino acid metabolism of archaea. [Display omitted] •Strengthening of a low-temperature anaerobic treatment process was evaluated.•Asparagine began to promote COD removal at asparagine 1 mmol/L.•Biochar and Fe(II) increased the activity of protease and coenzyme F420.•Biochar and Fe(II) enhanced bacterial amino acid metabolism.