Biological activity of silver nanoparticles synthesized using viticultural waste

• Published in: Microbial pathogenesis Vol. 190; p. 106613
• Main Authors: Miškovská, Anna, Michailidu, Jana, Kolouchová, Irena Jarošová, Barone, Ludovica, Gornati, Rosalba, Montali, Aurora, Tettamanti, Gianluca, Berini, Francesca, Marinelli, Flavia, Masák, Jan, Čejková, Alena, Maťátková, Olga
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2024
• Subjects: Animals; Anti-Infective Agents - chemistry; Anti-Infective Agents - pharmacology; Antibiofilm; Antimicrobial; Biofilms - drug effects; Bombyx; Cell Line; Cell Survival - drug effects; Cytotoxicity; Gram-Negative Bacteria - drug effects; Green Chemistry Technology; Humans; Insect model; Keratinocytes - drug effects; Larva - drug effects; Metal Nanoparticles - chemistry; Microbial Sensitivity Tests; Particle Size; Plant Extracts - chemistry; Plant Extracts - pharmacology; Silver - chemistry; Silver - metabolism; Silver - pharmacology; Silver nanoparticle; Vitis - chemistry; Vitis vinifera; Yeasts - drug effects; Antimicrobial; Insect model; Cytotoxicity; Antibiofilm; Vitis vinifera; Silver nanoparticle
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2024.106613

This research paper presents a novel approach to the green synthesis of silver nanoparticles (AgNPs) using viticultural waste, allowing to obtain NP dispersions with distinct properties and morphologies (monodisperse and polydisperse AgNPs, referred to as mAgNPs and pAgNPs) and to compare their biological activities. Our synthesis method utilized the ethanolic extract of Vitis vinifera pruning residues, resulting in the production of mAgNPs and pAgNPs with average sizes of 12 ± 5 nm and 19 ± 14 nm, respectively. Both these AgNPs preparations demonstrated an exceptional stability in terms of size distribution, which was maintained for one year. Antimicrobial testing revealed that both types of AgNPs inhibited either the growth of planktonic cells or the metabolic activity of biofilm sessile cells in Gram-negative bacteria and yeasts. No comparable activity was found towards Gram-positives. Overall, pAgNPs exhibited a higher antimicrobial efficacy compared to their monodisperse counterparts, suggesting that their size and shape may provide a broader spectrum of interactions with target cells. Both AgNP preparations showed no cytotoxicity towards a human keratinocyte cell line. Furthermore, in vivo tests using a silkworm animal model indicated the biocompatibility of the phytosynthesized AgNPs, as they had no adverse effects on insect larvae viability. These findings emphasize the potential of targeted AgNPs synthesized from viticultural waste as environmentally friendly antimicrobial agents with minimal impact on higher organisms. •Vitis vinifera pruning residues: an excellent source for green AgNPs synthesis.•AgNPs of different morphology are active against a broad spectrum of pathogens.•V. vinifera-derived AgNPs display anti-biofilm activity.•Polydisperse AgNPs exhibit a higher antimicrobial efficacy.•Effective concentrations of AgNPs show low cytotoxicity and in vivo toxicity.