Acetonitrile extraction coupled with UHPLC–MS/MS for the accurate quantification of 17 heterocyclic aromatic amines in meat products

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1068-1069; pp. 173 - 179
• Main Authors: Yan, Yan, Zhang, Shuang, Tao, Guan-jun, You, Feng-hui, Chen, Jie, Zeng, Mao-mao
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.11.2017
• Subjects: Acetonitrile extraction; Acetonitriles - chemistry; Amines - analysis; Amines - isolation & purification; Animals; Chromatography, High Pressure Liquid - methods; Heterocyclic aromatic amines; Heterocyclic Compounds - analysis; Heterocyclic Compounds - isolation & purification; Limit of Detection; Linear Models; Meat - analysis; Meat products; Reproducibility of Results; Swine; Tandem Mass Spectrometry - methods; UHPLC–MS/MS; UHPLC–MS/MS; Acetonitrile extraction; Heterocyclic aromatic amines; Meat products
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2017.10.015

•An acetonitrile method was proposed to detect 17 HAAs in meat products.•The new method obtained significant improvement of recoveries and repeatabilities.•All 17 HAAs had LOQs <0.648ng/g and proper linear ranges.•The new method showed adaptability in ten commercial products with high recoveries. This study proposed a simple and accurate acetonitrile extraction pretreatment method coupled with ultrahigh-performance liquid chromatography with tandem mass spectrometry for the simultaneous determination of 17 heterocyclic aromatic amines (HAAs) in meat products. With this new method, all 17 HAAs, including 11 polar and 6 nonpolarHAAs, were simultaneously extracted by acetonitrile and purified by one-step Oasis MCX cartridge purification. Compared with two different improved reference methods, the acetonitrile method could obtain higher recoveries (in the range of 42.5% to 99.0%) and better repeatability (lower than 12.2%). The limits of quantification were calculated between 0.028ngg−1and0.648ngg−1 with high correlation coefficients (r>0.9976) in wide linear ranges. The proposed acetonitrile method was successfully applied to the analysis of the HAAs levels in 10 commercial meat products with satisfactory recoveries.