Rapid miniprep isolation of mitochondrial DNA from metacestodes, and free-living and parasitic nematodes

A method, based on one to isolate supercoiled plasmid DNA from bacterial cells, has been developed to purify mitochondrial DNA (mtDNA) from cestode and nematode tissue easily and efficiently. Starting with as little as 100 mg of helminth tissue, sufficient mtDNA for electrophoretic analysis was extr...

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Bibliographic Details
Published inThe Journal of parasitology Vol. 79; no. 6; p. 964
Main Authors Peloquin, J J, Bird, D M, Platzer, E G
Format Journal Article
LanguageEnglish
Published United States 01.12.1993
Subjects
Animals
Caenorhabditis elegans - genetics
Centrifugation
Cestoda - genetics
Densitometry
DNA, Mitochondrial - isolation & purification
Female
Mermithoidea - genetics
Mice
Nematoda - genetics
Nucleic Acid Hybridization
Restriction Mapping
Taenia - genetics
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Summary:A method, based on one to isolate supercoiled plasmid DNA from bacterial cells, has been developed to purify mitochondrial DNA (mtDNA) from cestode and nematode tissue easily and efficiently. Starting with as little as 100 mg of helminth tissue, sufficient mtDNA for electrophoretic analysis was extracted. This DNA was essentially free of nuclear DNA and readily digested by restriction endonucleases. Approximately 20% of the mtDNA in helminth tissue was recovered, which is a significant improvement over previously available techniques.
ISSN:0022-3395
DOI:10.2307/3283741