Moxidectin residues in lamb tissues: Development and validation of analytical method by UHPLC-MS/MS

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1072; pp. 390 - 396
• Main Authors: Del Bianchi A. Cruz, Michelle, Fernandes, Maria A.M., de C. Braga, Patricia A., Monteiro, Alda L.G., Daniel, Daniela, Reyes, Felix G.R.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2018
• Subjects: Lamb tissues; Moxidectin; QuEChERS; Residues determination; UHPLC-MS/MS; Residues determination; Moxidectin; UHPLC-MS/MS; Lamb tissues; QuEChERS
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2017.11.041

•Novel method for determination of moxidectin residues in lamb tissues by UHPLC-ESI-MS/MS.•Single sample preparation step developed for moxidectin extraction from target tissues.•Incurred samples from all target tissues used in the validation step.•Short chromatographic run (5.0min) per sample.•Reliable high-throughput method for surveillance of moxidectin in animal tissue. The development and validation of a throughput method for the quantitation of moxidectin residues in lamb target tissues (muscle, kidney, liver and fat) was conducted using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS). To achieve higher recovery of the analyte from the matrices, a modified QuEChERS method was used for sample preparation. The chromatographic separation was achieved using a Zorbax Eclipse Plus C18 RRHD column with a mobile phase comprising 5mM ammonium formate solution +0.1% formic acid (A) and acetonitrile +0.1% formic acid (B) in a linear gradient program. Method validation was performed based on the Commission Decision 2002/657/EC and VICH GL49. To quantify the analyte, matrix-matched analytical curves were constructed with spiked blank tissues, with a limit of quantitation of 5ngg−1 and limit of detection of 1.5ngg−1 for all matrices. The linearity, decision limit, detection capability accuracy, and inter- and intra-day repeatability of the method are reported. The method was successfully applied to incurred lamb tissue samples (muscle, liver, kidney and fat) in a concentration range from 5 to 200μgkg−1, which demonstrated its suitability for monitoring moxidectin residues in lamb tissues in health surveillance programs, as well as for pharmacokinetics and residue depletion studies.