Application of cation-modified sulfur nanoparticles as an efficient sorbent for separation and preconcentration of carbamazepine in biological and pharmaceutical samples prior to its determination by high-performance liquid chromatography

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1063; pp. 245 - 252
• Main Authors: Ghoraba, Zahra, Aibaghi, Behzad, Soleymanpour, Ahmad
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.09.2017
• Subjects: Adsorption; Carbamazepine; Carbamazepine - analysis; Carbamazepine - chemistry; Carbamazepine - isolation & purification; Cations; Chromatography, High Pressure Liquid - methods; HPLC; Humans; Linear Models; Milk, Human - chemistry; Nanoparticles - chemistry; Reproducibility of Results; Sensitivity and Specificity; Solid phase extraction; Solid Phase Extraction - methods; Sulfur - chemistry; Sulfur nanoparticles; Tablets - chemistry; Solid phase extraction; Sulfur nanoparticles; Carbamazepine; HPLC
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2017.07.048

•Sulfur nanoparticles were proposed as an efficient sorbent for solid-phase extraction of carbamazepine.•The method shows high performance in all analytical parameters.•The method is potentially applicable for the determination of carbamazepine in biological samples.•Proposed Sorbent is stable in harsh acidic and basic conditions and reusable. A simple and rapid solid phase extraction procedure using a mini-column packed with modified sulfur nanoparticles as a new, efficient and reusable sorbent has been developed for the preconcentration of carbamazepine prior to its determination by high performance liquid chromatography. This method is based on the retention of carbamazepine by modified sulfur nanoparticles which are quite efficient for fast adsorption and desorption of carbamazepine. An HPLC system including C18, 250×4.6mm column, methanol-acidic water (pH=2.6 by acetic acid) (60:40) as mobile phase and UV detector (λ=276nm) was used. The effects of multiple experimental conditions such as the effect of pH, type and volume of buffer, type and volume of eluent, sample and eluent flow rate, sorbent amount and interfering ions, on the analytical performance of the method were investigated. The calibration curve was linear in the range of 0.5–200ngmL−1 and LOD of the proposed method was found to be 0.16ngmL−1. The procedure was successfully applied for the determination of carbamazepine in pharmaceutical samples, human plasma and breast milk.