Proteomic analysis reveals differential responsive mechanisms in Solanum nigrum exposed to low and high dose of cadmium

• Published in: Journal of hazardous materials Vol. 448; p. 130880
• Main Authors: Song, Ling-Yu, Liu, Xiang, Zhang, Lu-Dan, Hu, Wen-Jun, Xu, Chao-Qun, Li, Jing, Song, Shi-Wei, Guo, Ze-Jun, Sun, Chen-Yang, Tang, Han-Chen, Wang, Ji-Cheng, Zhu, Xue-Yi, Zheng, Hai-Lei
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.04.2023
• Subjects: Biodegradation, Environmental; Cadmium - metabolism; Differentially expressed proteins (DEPs); Hyperaccumulator; Plant Roots - metabolism; Protein-protein interaction (PPI) network; Proteome; Proteomics; Soil; Soil Pollutants - metabolism; Solanum nigrum - metabolism; Differentially expressed proteins (DEPs); Cd; Proteome; Protein-protein interaction (PPI) network; Hyperaccumulator
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2023.130880

Cadmium (Cd) contamination is becoming a widespread environmental problem. However, the differential responsive mechanisms of Cd hyperaccumulator Solanum nigrum to low or high dose of Cd are not well documented. In this study, phenotypic and physiological analysis firstly suggested that the seedlings of S. nigrum showed slight leaf chlorosis symptoms under 25 μM Cd and severe inhibition on growth and photosynthesis under 100 μM Cd. Further proteomic analysis identified 105 differentially expressed proteins (DEPs) in the Cd-treated leaves. Under low dose of Cd stress, 47 DEPs are mainly involved in primary metabolic processes, while under high dose of Cd stress, 92 DEPs are mainly involved in photosynthesis, energy metabolism, production of phytochelatin and reactive oxygen species (ROS). Protein-protein interaction (PPI) network analysis of DEPs support above differential responses in the leaves of S. nigrum to low and high dose of Cd treatments. This work provides the differential responsive mechanisms in S. nigrum to low and high dose of Cd, and the theoretical foundation for the application of hyperaccumulating plants in the phytoremediation of Cd-contaminated soils. [Display omitted] •Low dose of Cd induces chlorosis, high dose of Cd inhibits growth of S. nigrum.•105 differentially expressed proteins (DEPs) were identified under CdCl2 treatment.•Low dose of Cd induces 47 DEPs, while high dose of Cd induces 92 DEPs.•Low dose of Cd is inclined to induce the changes in metabolic processes.•High dose of Cd inhibits photosynthesis and productions of phytochelatin and ROS.