Phosphine gas in the dark induces severe phytotoxicity in Arabidopsis thaliana by increasing a hypoxia stress response and disrupting the energy metabolism: Transcriptomic approaches

Phosphine (PH3) is an ideal fumigant alternative on methyl bromide (MB) as MB has been classified as an ozone-depleting substance. However, several challenges limit its efficient use in crop production, including the emergence of PH3-resistant insect pests and the incidence of phytotoxic effects on...

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Published inJournal of hazardous materials Vol. 443; no. Pt A; p. 130141
Main Authors Kim, Kyeongnam, Kim, Chaeeun, Yoo, Jinsung, Kim, Jun-Ran, Kim, Yoon-Ha, Lee, Sung-Eun
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 05.02.2023
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Summary:Phosphine (PH3) is an ideal fumigant alternative on methyl bromide (MB) as MB has been classified as an ozone-depleting substance. However, several challenges limit its efficient use in crop production, including the emergence of PH3-resistant insect pests and the incidence of phytotoxic effects on nursery plants. Therefore, this study aims to elucidate the mechanism underlying PH3 phytotoxicity in plants using transcriptomic techniques. Fumigation with 2 g/m3 PH3 induced phytotoxic effects in A. thaliana, as evidenced by a decrease in growth and vegetation indices compared to the control group. Transcriptomic analysis revealed that PH3 fumigation phytotoxicity responses in A. thaliana involve genes related to hypoxia stress and energy metabolism. Additionally, pretreatment with ethylene induced pre-adaptation to hypoxia under light conditions during fumigation effectively suppressed the phytotoxic effects of PH3 in A. thaliana by increasing the expression of hypoxia-adaptive genes. Moreover, the phytotoxicity of PH3 was also confirmed in pumpkin (Cucurbita moschata Duch.), and was dependent on light. Overall, our findings showed that fumigation under light conditions and ethylene pretreatment could be used to minimize PH3-induced phytotoxic effects in plants. [Display omitted] •Transcriptomic analysis revealed the toxic mechanism of PH3 using A. thaliana.•The phytotoxicity of PH3 was correlated with hypoxia stress and energy metabolism.•As per the light conditions, PH3 showed an opposite phytotoxic pattern.•PH3 had the property of inducing hypoxia adaptive genes like ethylene.
ISSN:0304-3894
1873-3336
DOI:10.1016/j.jhazmat.2022.130141