Selective inhibition of Leishmania donovani by active extracts of wild mushrooms used by the tribal population of India: An in vitro exploration for new leads against parasitic protozoans

• Published in: Experimental parasitology Vol. 138; pp. 9 - 17
• Main Authors: Mallick, Suvadip, Dutta, Aritri, Dey, Somaditya, Ghosh, Joydip, Mukherjee, Debarati, Sultana, Sirin Salma, Mandal, Supratim, Paloi, Soumitra, Khatua, Somanjana, Acharya, Krishnendu, Pal, Chiranjib
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2014
• Subjects: Agaricales - chemistry; Animals; Anti-leishmanial; Apoptosis; Complex Mixtures - pharmacology; Complex Mixtures - toxicity; Dendritic Cells - drug effects; Dendritic Cells - immunology; Hydrophobic and Hydrophilic Interactions; IL-12; India; Inhibitory Concentration 50; Interleukin-12 - metabolism; Leishmania donovani - drug effects; Leishmania donovani - growth & development; Lipid synthesis; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - metabolism; Macrophages, Peritoneal - parasitology; Medicine, Traditional; Mice; Mice, Inbred BALB C; Mushroom extract and fraction; Nitric oxide; Nitric Oxide - metabolism; Spleen - cytology; Spleen - drug effects; Termitomyces - chemistry; Tricholoma - chemistry; India; Mushroom extract and fraction; Lipid synthesis; Anti-leishmanial; IL-12; Nitric oxide
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/j.exppara.2014.01.002

Identified mushrooms used in folklore of Santal tribal populations in West Bengal, India. (a) Russula albonigra, (b) Russula laurocerasi, (c) Russula delica, (d) Termitomyces eurhizus, (e) Tricholoma giganteum, (f) Astraeus hygrometricus. •We assessed 18 mushroom extracts against L. donovani promastigote and amastigote.•A. hygrometricus and T. giganteum were found efficient against promastigotes.•A. hygrometricus and R. laurocerasi were found effective against amastigotes.•The active extracts were found to induce IL-12 and nitric oxide.•The active extracts were found considerably non-toxic to murine splenocytes. The study was intended at evaluating the anti-proliferating effect of mushrooms used in traditional folklore of Santal tribal population in India against Leishmania donovani (MHOM/IN/83/AG83). A total of eighteen extracts, three estracts from each mushroom [(80% ethanol extracted; Fa), (water-soluble polysaccharide fraction; Fb), (polyphenolic fraction; Fc)], from six wild mushrooms were obtained. These extracts were tested against the promastigotes and amastigotes for their antileishmanial capacity. Fa fractions (250μg/mL) of Astraeus hygrometricus and Tricholoma giganteum significantly inhibited the growth of L. donovani promastigotes and interfered in lipid biosynthesis. Moreover, both fractions induced apoptosis in promastigotes. Water soluble Fb fractions of A. hygrometricus, Russula laurocerasi, Russula albonigra, Termitomyces eurhizus, Russula delica and polyphenolic Fc fraction of R. laurocerasi were found to inhibit the replication of intracellular amastigotes in macrophages dose dependently. Significantly, 50% inhibitory concentration of the active extracts against intracellular amastigotes induced release of nitric oxide and IL-12 in murine macrophages and dendritic cells assay and also found considerably non-toxic on murine splenocytes. Results of this study can be used as a basis for further phytochemical and pharmacological investigations in the effort for search of novel anti-leishmanial leads.