Development of an interspecies interaction model: An experiment on Clostridium cadaveris and Clostridium sporogenes under anaerobic condition

• Published in: Journal of environmental management Vol. 237; pp. 247 - 254
• Main Authors: Koo, Taewoan, Lee, Joonyeob, Hwang, Seokhwan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2019
• Subjects: Biokinetic analysis; Clostridium cadaveris; Clostridium sporogenes; Interspecific interaction model development; Primer and probe set; Quantitative real-time PCR; Clostridium sporogenes; Quantitative real-time PCR; Interspecific interaction model development; Clostridium cadaveris; Biokinetic analysis; Primer and probe set
• ISSN: 0301-4797; 1095-8630
• DOI: 10.1016/j.jenvman.2019.02.084

The specific primer and probe sets for quantifying Clostridium cadaveris and Clostridium sporogenes using a quantitative real-time PCR were designed. Each primer and probe set detected only the target species very specifically. The two species were cultivated in pure and mixed culture in batch mode with glucose as the only carbon source. The designed QPCR sets were used successfully to estimate the biokinetic parameters of each species in pure culture: i.e., maximum specific growth rate μmax, half saturation concentration Ks, growth yield Y, and decay coefficient Kd. of C. cadaveris and C. sporogenes were 0.311 ± 0.020 and 0.360 ± 0.019 h-1, 4.241 ± 1.653 and 5.171 ± 1.097 g/L, 0.301 ± 0.065 and 0.199 ± 0.037 1011 copies/g, 0.005 ± 0.043 and 0.009 ± 0.025 h-1, respectively. The effect of interspecific interaction of on substrate consumption rate and microbial growth was evaluated using mixed culture; curve fitting and comparison of coefficients detected increase in substrate consumption rate but decrease in microbial growth rate; these results imply interspecific interaction effect. A new model was of the interspecific interaction was developed, with focus on accuracy, realism, simplicity and biological significance. This interspecific interaction model may be extended to more-complex bioprocesses such as biological wastewater treatment systems and anaerobic digestion. •The real-time PCR primer and probe sets of C. cadaveris and C. sporogenes were designed.•Biokinetic parameters derived from pure culture had statistically significant values.•Cadaveris and C. sporogenes were cultivated together to prove interactions effect.•Substrate consumption rate increased, while microbial growth rate decreased by interaction.•The novel interaction model was developed to quantify the interspecific interaction.