Chitosan coated nanostructured lipid carriers for enhanced in vivo efficacy of albendazole against Trichinella spiralis

• Published in: Carbohydrate polymers Vol. 232; p. 115826
• Main Authors: Eid, Rania K., Ashour, Dalia S., Essa, Ebtessam A., El Maghraby, Gamal M., Arafa, Mona F.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.03.2020
• Subjects: Chitosan; Intestinal absorption; Lipid nanostructure; Nanoparticles; Oral bioavailability; Nanoparticles; Lipid nanostructure; Oral bioavailability; Chitosan; Intestinal absorption
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2019.115826

[Display omitted] •Nanostructured lipid carriers are promising for oral delivery of albendazole in treatment of trichinellosis.•Chitosan coating of nanostructured lipid carriers fortifies the efficacy probably due to enhanced bioadhesion.•Drug release is not responsible for enhanced efficacy of albendazole from nanostructured lipid carriers.•Nanostructured lipid carriers enhance albendazole efficacy irrespective to the phase of infection.•None of the treatment protocols was able to achieve complete eradication of infection and multiple dosing is recommended. The study investigated chitosan coated nanostructured lipid carriers (NLCs) for oral delivery of albendazole in treatment of trichinellosis. NLCs comprised precirol and oleic acid with Tween and Span 80. Dicetylphosphate was used as charging agent to allow chitosan coating. Trichinella spiralis infected mice were used and albendazole suspension, coated or uncoated NLCs were orally administered at different stages of infection. NLCs were spherical with size of 188 and 200 nm for coated and uncoated NLC, respectively. Treatment during intestinal phase reduced worm count with NLCs showing better rank. This was reflected further by reduced larvae count and improved histopathological features. Starting treatment in the migrating phase reduced larval count by 62.9, 99.6 and 89.5 % after administration of suspension, coated and uncoated NLCs, respectively. The same rank was recorded for the encysted phase. NLCs enhanced the efficacy of albendazole against Trichinella spiralis compared with suspension with chitosan coated NLCs being superior.