Identification of wheat blue dwarf phytoplasma effectors targeting plant proliferation and defence responses

• Published in: Plant pathology Vol. 67; no. 3; pp. 603 - 609
• Main Authors: Wang, N., Li, Y., Chen, W., Yang, H. Z., Zhang, P. H., Wu, Y. F.
• Format: Journal Article
• Language: English
• Published: Oxford Wiley Subscription Services, Inc 01.04.2018
• Subjects: Apoptosis; BAX protein; callose; Cell death; China; dwarfing; Dwarfism; Effectors; gene expression regulation; genetic markers; Hydrogen peroxide; Hypersensitive response; Immunity; Leaves; microorganisms; Nicotiana benthamiana; Pathogenesis; pathogenesis-related proteins; Phytoplasma; Plant immunity; Proteins; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; SAP11‐like; TENGU‐like; Tips; Virulence; Wheat; wheat blue dwarf phytoplasma; Witches' broom; Yellow leaf; China
• ISSN: 0032-0862; 1365-3059
• DOI: 10.1111/ppa.12786

The identification of effectors from pathogenic microbes is one of the most important subjects for elucidating infection mechanisms. Wheat blue dwarf (WBD) phytoplasma causes dwarfism, witches' broom, and yellow leaf tips in wheat plants, resulting in severe yield loss in northwestern China. In this study, 37 candidate effector proteins were transiently expressed in Nicotiana benthamiana. Plants expressing the SAP11‐like protein SWP1 exhibited typical witches' broom. Interestingly, another protein, SWP11, induced both cell death and defence responses, including H2O2 accumulation and callose deposition. Analysis by qRT‐PCR was used to show that a marker gene of the hypersensitive response, HIN1, and three pathogenesis‐related genes, PR1, PR2 and PR3, were significantly up‐regulated in leaves of N. benthamiana expressing SWP11. In addition, SWP12 and SWP21 (TENGU‐like) were shown to suppress SWP11‐, BAX‐, and/or INF1‐induced cell death. These results indicated that SWP21 has a distinct role in virulence compared with TENGU and that WBD phytoplasma possesses effectors that target plant proliferation and defence responses. The ability of these effectors to trigger or suppress plant immunity provides new insights into the phytoplasma–plant interaction.