Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX

A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In...

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Published inNucleic acids research Vol. 11; no. 8; pp. 2325 - 2335
Main Authors Jaye, Michael, Salle, Henri de la, Schamber, Fabienne, Balland, Alain, Kohli, Vipin, Findeli, Annie, Tolstoshev, Paul, Lecocq, Jean-Pierre
Format Journal Article
LanguageEnglish
Published England Oxford University Press 25.04.1983
Subjects
Alanine
Amino Acid Sequence
Animals
Base Sequence
Cattle
Cloning, Molecular
Codon - genetics
DNA - isolation & purification
Factor IX - genetics
Hemophilia A - genetics
Humans
Liver - metabolism
Molecular Biology
Oligodeoxyribonucleotides
Oligonucleotides
Species Specificity
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Summary:A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In addition, precisely one codon has been deleted.
Bibliography:ArticleID:11.8.2325
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ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/11.8.2325