Effects of nitric oxide synthase inhibitor on mitochondria apoptosis and meat quality in postmortem Gannan yak (Bos grunniens) meat

• Published in: Journal of food biochemistry Vol. 46; no. 9; pp. e14234 - n/a
• Main Authors: Tian, Zhu, Li, Xueru, Shi, Xixiong, Chen, Cheng
• Format: Journal Article
• Language: English
• Published: United States 01.09.2022
• Subjects: Gannan yak meat; L‐NAME; meat quality; mitochondria apoptosis; postmortem aging; Gannan yak meat; meat quality; postmortem aging; L-NAME; mitochondria apoptosis
• ISSN: 0145-8884; 1745-4514
• DOI: 10.1111/jfbc.14234

This research aimed to explore the effects of the nitric oxide synthase (NOS) inhibitor (L‐NAME) on mitochondria apoptosis in postmortem Gannan yak (Bos grunniens) longissimus dorsi (LD) muscle and to explore its effect on meat quality further. The Gannan yak meat samples were treated with the control group (0.9% NaCl) and L‐NAME (20, 60, and 100 mM) for 24 h and then stored for 0, 1, 3, 5, and 7 days at 4°C. NOS activity and NO content were investigated, and the parameters of mitochondrial apoptosis of the postmortem Gannan yak meat were determined. Meanwhile, the meat quality such as the centrifugation loss, meat color, and myofibril fragmentation index (MFI) was evaluated. The results indicated that after treatment with L‐NAME, NOS activity and NO content decreased, causing mitochondrial membrane damage, Bax protein, and Cyt‐c levels increased, and resulted in increased activities of caspase‐9 and ‐3, promoting the occurrence of mitochondrial apoptosis. Furthermore, it increased the tenderness and water retention of Gannan yak meat. The results indicated that NOS inhibitor played a regulatory role in postmortem Gannan yak meat quality by regulating mitochondria apoptosis during postmortem aging. Practical applications The meat's tenderness is often considered the most important factor affecting consumers' willingness to repurchase. The relationship of caspases and MFI suggested that L‐NAME played a regulatory role in postmortem Gannan yak meat quality by regulating mitochondria apoptosis during postmortem aging. This study provides valuable information for the development of the Gannan yak economy in Tibetan areas. Kidney bean cysteine protease was characterized as a major cross‐reacting allergen by in silico and in vitro methods. In computational assessment cross‐reactivity and allergenicity of allergen was determined using sequence homology approach. This was followed by cloning and purification of allergen using recombinant DNA technology and affinity chromatography, respectively. Finally, in vitro analysis using food hypersensitive patients' sera validated in silico data, thereby representing cysteine protease as a potential allergen.