Culture of mouse prostatic epithelial cells from genetically engineered mice

BACKGROUND The lack of appropriate prostate cancer models is a major problem for prostate cancer research. Progress has been made towards the development of better in vivo rodent genetic models for prostatic disease. However, an in vitro model is often preferred for the elucidation of cellular mecha...

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Bibliographic Details
Published inThe Prostate Vol. 63; no. 3; pp. 291 - 298
Main Authors Barclay, Wendy W., Cramer, Scott D.
Format Journal Article
LanguageEnglish
Published Hoboken Wiley Subscription Services, Inc., A Wiley Company 15.05.2005
Subjects
Animals
Cell Culture Techniques
Cell Cycle Proteins - genetics
Cell Division
Cells, Cultured
Cre
Culture Media, Serum-Free
Cyclin-Dependent Kinase Inhibitor p21
Disease Models, Animal
Epithelial Cells - cytology
Fluorescent Antibody Technique
Genotype
Humans
Immunoblotting
Male
Mice
Mice, Knockout
Microscopy, Fluorescence
Prostate - cytology
prostate cancer
Prostatic Diseases - genetics
Receptors, Androgen - genetics
Receptors, Calcitriol - deficiency
Receptors, Calcitriol - genetics
three-dimensional culture
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Summary:BACKGROUND The lack of appropriate prostate cancer models is a major problem for prostate cancer research. Progress has been made towards the development of better in vivo rodent genetic models for prostatic disease. However, an in vitro model is often preferred for the elucidation of cellular mechanisms involved in the disease. METHODS We microdissected the four prostatic lobes from young male mice, harvested the epithelial components, and grew epithelial cells from these tissues. We maintained the growth of these cells in long‐term and three‐dimensional culture. RESULTS We have reproducibly harvested and cultured for extended passages mouse prostatic epithelial cells (MPECs) from a variety of mouse genetic strains. These cells express luminal and basal epithelial markers as well as the androgen receptor. Additionally, MPECs form classic branching structures in a three‐dimensional collagen matrix. CONCLUSIONS We have developed a novel culture system to harvest and grow MPECs in long‐term culture. These cells will serve as a useful in vitro complement to studies using mouse genetic models for prostatic disease. © 2004 Wiley‐Liss, Inc.
Bibliography:ArticleID:PROS20193
istex:9A86E5F0019865FFB448434219EB6057F74563EE
National Cancer Institute (to W.W.B.) - No. T32 CAO79448
ark:/67375/WNG-6FN4TRSQ-W
Kulynych Cancer Research Fund
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0270-4137
1097-0045
DOI:10.1002/pros.20193