Nitrogen supply alleviates seed yield reduction by improving the morphology and carbon metabolism of pod walls in shaded rapeseed

• Published in: Physiologia plantarum Vol. 175; no. 5; pp. e14003 - n/a
• Main Authors: Kuai, Jie, Nie, Xiaoyu, Lou, Hongxiang, Li, Zhen, Xie, Xiongze, Sun, Yingying, Xu, Zhenghua, Wang, Jing, Wang, Bo, Zhou, Guangsheng
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.2023; Wiley Subscription Services, Inc
• Subjects: Carbon; Chlorophyll; Chloroplasts; Crop yield; Elongation; Flowering; Fructose; Glucose; Light transmission; Luminous intensity; Metabolism; Morphology; Nitrogen; Photosynthesis; Plants (botany); Rapeseed; Ribulose-bisphosphate carboxylase; Seeds; Shading; Sucrose; Sucrose phosphate synthase; Sucrose synthase; Ultrastructure; Weight
• ISSN: 0031-9317; 1399-3054
• DOI: 10.1111/ppl.14003

Shading significantly affects rapeseed yield, while reasonable nitrogen (N) application has efficiency gains. However, the functions and mechanisms of N are not fully established for shaded rapeseed plants. Therefore, we conducted a 2‐year field experiment to study the effect of N on pod wall morphology and carbon metabolism of shaded rapeseed. Two varieties, three N rates (120 [N1], 240 [N2], and 360 [N3] kg hm−2) and two light intensities (100 and 70% light transmission) from 10 to 35 days after the end of flowering were set as experimental parameters. Shading decreased the pod wall chlorophyll content, ribulose 1,5‐bisphosphate carboxylase (Rubisco) activity and glucose content at 25 and 35 days after flowering (DAF). Decreased sucrose synthase (SuSy) and sucrose phosphate synthase activity caused by shading reduced sucrose and fructose content. They are responsible for the decline in the 1000‐seed weight and a 22.1–37.6% decline in seed yield. More N under shading promoted pod elongation and pigment content, improved chloroplast ultrastructure, increased Rubisco and SuSy activity at 35 DAF, thus contributing to pod wall photosynthesis and fructose and glucose levels in shaded rapeseed plants. Similar trends were observed in pod number, pod weight, and seed weight, while the greatest increase in seed/wall ratio was observed under N2 for shaded rapeseed plants. The results indicated that N can reduce the yield difference between different light conditions and balance partitioning and conversion of photoassimilates in pod wall, but avoid applying an excessive amount of nitrogen.